Research Facility : Immunology Core Laboratory
The Immunology Core will serve as a core facility to provide expertise for the incorporation of the emerging technologies to support immunological assays for clinical and pre-clinical research, and offer expert scientific and technical consultation to investigators conducting basic and translational research.
Service — A one stop state-of-the-art facility for immunophenotyping, functional assays to support vaccine and drug candidate's clinal and pre-clinical studies.
Education — Immunology Core lab will provide training and mentor staff, students, research fellows, and investigators in immunology research, to stimulate inclusion of immunology research in new studies through consultations and educational initiatives that inform the community of recent advances in immunology, and to help community members to understand and interpret research findings.
Research — Immunology Core lab will develop, optimize, and validate specialized immunological assays to accelerate research in the prevention, treatment, and monitoring of diseases.
Funding – The initial funding for inception of Immunology Core lab was provided by the THSTI Core Grant. In the meantime, the Immunology Core lab received the COVID Suraksha Grant REOI-2 (BT/CS0010/CS/02/20) from DBT-BIRAC as "A cellular assay platform for determining the immunogenicity of vaccine candidates.“
The immunology Core lab at THSTI provides a one-stop solution for all the internationally accepted cellular immune assays, such as:
1.Thiruvengadam R, Awasthi A, Medigeshi G, Bhattacharya S, Mani S, Sivasubbu S, Shrivastava T, Samal S, Murugesan DR, Desiraju BK, Kshetrapal P. Effectiveness of ChAdOx1 nCoV-19 vaccine against SARS-CoV-2 infection during the delta (B. 1.617. 2) variant surge in India: a test-negative, case-control study and a mechanistic study of post-vaccination immune responses. The Lancet Infectious Diseases. 2021 Nov 25.
2.Thiruvengadam R, Binayke A, Awasthi A*. SARS-CoV-2 delta variant: A persistent threat to the effectiveness of vaccines. The Lancet Infectious Diseases. 2022 Mar 1;22(3):301-2.
3.Binayke A, Zaheer A, Dandotiya J, Gupta SK, Mani S, Tripathy MR, Madan U, Shrivastava T, Kumar Y, Pandey AK, Rathore DK, Awasthi A*. Innate immune response and distinct metabolomic signatures together drive and shape the SARS-CoV-2-specific T cell response during COVID-19. bioRxiv. 2022 Jan 1.
4.Thuluva, Subhash, Vikram Paradkar, Kishore Turaga, Subbareddy Gunneri, Vijay Yerroju, Rammohan Reddy Mogulla, Venkata Suneetha Pothakamuri et al. "Immunogenic superiority and safety of Biological E CORBEVAX vaccine compared to COVISHIELD (ChAdOx1 nCoV-19) vaccine studied in a phase III, single blind, multicenter, randomized clinical trial." medRxiv (2022).
5.Rizvi ZA, Dalal R, Sadhu S, Binayke A, Dandotiya J, Kumar Y, Shrivastava T, Gupta SK, Aggarwal S, Tripathy MR, Rathore DK, Awasthi A*. Golden Syrian hamster as a model to study cardiovascular complications associated with SARS-CoV-2 infection. Elife. 2022 Jan 11;11:e73522.
6.Rizvi ZA, Tripathy MR, Sharma N, Goswami S, Srikanth N, Sastry JL, Mani S, Surjit M, Awasthi A*, Dikshit M*. Effect of Prophylactic Use of Intranasal Oil Formulations in the Hamster Model of COVID-19. Frontiers in pharmacology. 2021;12.
7.Rizvi ZA, Sadhu S, Dandotiya J, Binayke A, Sharma P, Singh V, Das V, Khatri R, Kumar R, Samal S, Kalia M, Awasthi A*. SARS-CoV-2 and its variants, but not Omicron, induces thymic atrophy and impaired T cell development. bioRxiv. 2022 Jan 1.
8.Thuluva, S., et al. (2022). "Safety, tolerability and immunogenicity of Biological E’s CORBEVAX™ vaccine in children and adolescents: A Prospective, Randomised, Double-blind, Placebo controlled, Phase-2/3 Study." medRxiv: 2022.2004.2020.22274076.
In the past two years of pandemic, the Immunology Core Lab is working round the clock to answer the questions of national importance and help our nation contain the COVID-19 pandemic at the earliest. COVID-19 vaccine pre-clinical Trials supported by Immuno-biology Lab and Immunology Core Lab at THSTI:
Following are some of the contract services provided for different vaccine clinical trials:
Future Pandemic Preparedness
One of the long-term goals of Immunology Core Lab is to develop robust infrastructure and scientific know-how capable of rapid escalation of resources to study cellular immunogenicity for any future health emergency. Following are some of the aspects Immunology Core Lab is working on: