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DR. DEEPAK KUMAR RATHORE, PhD  

Senior Research Scientist
+91-129-2876355 
drathore [at] thsti [dot] res [dot] in
Research Officer, Translational Health Science and Technology Institute, Faridabad
Post-doctoral Project Associate from National Institute of Immunology, New Delhi
Ph.D. in Biochemistry from Jiwaji University, Gwalior, M.P.
M.Sc. in Biochemistry from Jiwaji University, Gwalior, M.P.


Area of works:

Leading Flowcytometry core facility

Immunophenotyping and Immunobiology

Research Interest:

  • Comprehensive Immunophenotyping in Healthy Individuals vs Disease Conditions.
  • Understanding the problem of low birth weight neonates born with full gestational age.

 

Research Work Areas Summary:

1. Human Neonatal Biology (Immunophenotyping/ Flowcytometry): Our approaches involved immunophenotypic characterization of umbilical cord blood cells in full-term appropriate and small for gestational age neonates using well standardize flowcytometry method. In our efforts to correlates the frequency of immune cells including T cells, B cells, NK cells, monocytes, neutrophils and dendritic cells and subsets of most of cell types with the birth weight and gestational age of babies.  Another approach to evaluate the immune cells indian neonates with overseas and identified the cellular composition of Indian and USA neonates.

2. Hemolytic Disease (Hematological disorders): One of collaborative approach as working on circulating monocytes (CD14+) develop a highly pro-inflammatory (CD14+CD16hi) and procoagulant phenotype when they engulf Hb-activated platelets, and are abundant in patients of PNH and SCD. These cells upon engulfment of Hb-activated platelets, monocytes differentiate into M1- macrophages.

3. Immunobiology: Another collaborative worked on IL-9 induction in helper T cells and we showed that Foxo1 is differentially expressed in Th9 cells, and is required for the induction of IL-9 in Th2, Th9, Th17 and iTreg cells.

4. Animal Parasite Immunology: Identification purification and characterization of immunogenic excretory/secretory protein from the parasitic nematode Haemonchus contortus of goat and sheep its interaction with host blood cells, which has vast implications in animal husbandry and in understanding host parasite relationship.

 

 

 


 

Research Papers:

1.   Singhal R*, Rathore DK*, Bhakuni T, Seth Rand Guchhait P (2019). Absence of non-classical monocytes in patients with PNH or SCD: free Hb and NO mediated mechanism. Journal of Immunology Research. Doi.org/10.1155/2019/1409383(*Equal first author). (Impact factor 3.4)

2.   Rathore DK, HolmesTH,Nadeau K, Mittal P, Batra A, Hasson YR, Sopory S, Gupta R, Chellani HK, Aggarwal KC, Bal V, Natchu UCM, Bhatnagar S, Tavassoli M, Lyell DJ, Rath S, Wadhwa N and MaeckerHT,  (2018).Differences in cord blood B-1 cells and other immune parameters between Indian and US Infants. PLOS ONE13(11):e0207297. PMID: 30444901 (Impact factor 2.8)

3.   Singhal R, Chawla S, Batra H, Gupta S, Ojha A, Rathore DK and Guchhait P (2018). Monocytes engulf Hb-activated platelets are differentiated into pro-inflammatory macrophages in hemolytic conditions. European Journal of Immunology. doi: 10.1002/eji.201747449. PMID: 29677388 (Impact factor 4.3)

4.   Malik S, Sadhu S, Elesela S, Pandey RP, Chawla AP, Sharma D, Panda L, Rathore D, Ghosh B, Ahuja V and Awasthi A. (2017). Transcription factor Foxo1 is essential for IL-9 induction in T helper cells. Nature Communications Oct 9;8(1):815. doi: 10.1038/s41467-017-00674-6.PMID: 28993609 (Impact factor 12.0)

5.   Singhal R, Chawla S, Rathore DK*, Bhasym A, Annarapu GK, Sharma V, Seth R and Guchhait P (2017). Development of pro-inflammatory phenotype in monocytes after engulfing Hb-activated platelets in hemolytic disorders. Clinical Immunology175:133-142.PMID: 28039017 (*Second author) (Impact factor 4.0)

6.   Prabhu SB, Rathore DK, Nair D, Chaudhary A, Raza S, Kanodia P, Sopory S, George A,  Rath S, Bal V, Tripathi R, Ramji S, BatraA, Aggarwal K C, Chellani HK, Arya S, Bhatla N, Paul VK, Aggarwal R, Agarwal N, Mehta U, Natchu UCM, WadhwaN and Bhatnagar S (2016). Comparison of human neonatal and adult blood leukocyte subset composition phenotypes. PLoS ONE 11(9):e0162242.PMID: 27610624 (Impact factor 3.8)

7.   Rathore DK, Nair D, Raza S, Saini S, Singh R, Kumar A, Tripathi R, Ramji S, Batra A,Aggarwal KC, Chellani HK,Arya S, Bhatla N, Paul VK, Aggarwal R, Agarwal N, Mehta U, Sopory S, Natchu UCM, Bhatnagar S, Bal V, Rath S and WadhwaN (2015). Underweight full-term Indian neonates show differences in umbilical cord blood leukocyte- Acorss sectional study. PLoS ONE, 10(4):e0123589.PMID: 25898362 (Impact factor 3.8)

8.   Shenoy GN, Chatterjee P, Kaw S, Mukherjee S, Rathore DK*, Bal V, Rath S and George A (2012). Recruitment of memory B cells to lymph nodes remote from the site of immunization requires an inflammatory stimulus. Journal of Immunology,189: 521-528. PMID: 22675203 (*Second author) (Impact factor 5.8)

9.   Suchitra S, Anbu KA, Rathore DK, Mahawar M, Singh BP and Joshi P (2008). Haemonchus contortus calreticulin binds to C-reactive protein of its host, a novel survival strategy of the parasite. Parasite Immunology,30:371-374. PMID: 18422872 (Impact factor 2.8)

10.  Rathore DK, Suchitra S, Saini M, Singh BP and Joshi P (2006). Identification of 66kDa Haemonchus contortus excretory/secretory antigen that inhibits host monocytes. Veterinary Parasitology, 138: 291-300. PMID: 16517075 (Impact factor 2.6)

Kumar N, Sharma C, Chaudhuri S, Rathore DK, Kumar S, Haldar S, Pillai J, Bhatnagar S. “Flow-cytometry based rapid method of testing antimicrobial drug susceptibility and resistance in biological fluids”. (TEMP/E-1/48033/2017-DEL; filed December 28, 2017)

  • Executive Council Member of Indian Immunology Society.
  • Life membership of Society for Immunology & Immunopathology.
  • The Cytometry Society (India).
  • Life Membership of Indian Immunology Society.

Awards:

  • Awatar Krishan prize in the 14th Indo-US Cytometry Workshop at Institute of Life Sciences, Bhubaneswar, India.
  • The best poster presentation award in the 3rd National Extension Education Congress at N.D.R.I. Karnal, India.
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