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DR. GURUPRASAD R. MEDIGESHI, Ph.D.  

Professor
+91-129-2876311  (+91-129-6525537)
gmedigeshi [at] thsti [dot] res [dot] in
Ph.D. from Georg-August University, Goettingen, Germany.
M.Sc. Biotechnology, University of Mysore, Karnataka, India.
B.Sc., Yuvaraja College, University Of Mysore, Karnataka, India.


“Accepting PhD students with CSIR-UGC/DBT/DST/ICMR fellowships” | New publication: https://www.frontiersin.org/articles/10.3389/fmolb.2020.586254/full


https://www.indiascience.in/videos/covid-19-warriors-life-in-science-with-pallava-bagla-e

 

 

Present Research Interest

Majority of childhood infections in India are viral in nature and most of these infections cannot be prevented by vaccines or treated by commercial drugs. Our group primarily focuses on the host-pathogen interactions in human viral infections with particular focus on flaviviruses (Ex: Dengue virus) and respiratory viruses (Ex: Respiratory Syncytial Virus). Our primary objective is to generate basic knowledge in virus-host interactions that can be harnessed for vaccine and drug development. Focus areas include:

  • Identifying cellular signaling pathways and protein trafficking components that play an essential role at various stages of viral life-cycle
  • Understanding the interactions between cellular permeability barriers and viral infections
  • Identifying host and viral factors that influence disease outcomes in patients
  • Using high-throughput screening and in vitro assays to identify novel scaffolds for antiviral development and also for repurposing drugs

Collaborations

  • National Institute of Immunology, New Delhi
  • National Brain Research Center, Manesar
  • International Center for Genetic Engineering and Biotechnology, New Delhi
  • Indian Institute of technology, New Delhi
  • All India Institute of Medical Sciences, New Delhi
  • India Institute of Science, Bangalore
  1. Kumar P, Mukherjee A, Randev S, Medigeshi GR, Jat KR, Kapil A, Lodha R, Kabra SK. Epidemiology of Coronavirus Infection in Children and Their Impact on Lung Health: Finding from a Birth Cohort Study. Pediatr Infect Dis J. 2020. Epub 2020/09/15. doi: 10.1097/INF.0000000000002884. PubMed PMID: 32925544.
  2. Kumar P, Mukherjee A, Randev S, Medigeshi GR, Jat KR, Kapil A, Lodha R, Kabra SK. Effect of acute respiratory infections in infancy on pulmonary function test at 3 years of age: a prospective birth cohort study. BMJ Open Respir Res. 2020;7(1). Epub 2020/02/23. doi: 10.1136/bmjresp-2019-000436. PubMed PMID: 32079606; PMCID: PMC7047475.
  3. Khan NA, Singla M, Samal S, Lodha R, Medigeshi GR. Respiratory Syncytial Virus-Induced Oxidative Stress Leads to an Increase in Labile Zinc Pools in Lung Epithelial Cells. mSphere. 2020;5(3). Epub 2020/05/29. doi: 10.1128/mSphere.00447-20. PubMed PMID: 32461278; PMCID: PMC7253603.
  4. Icmr Covid Study Group CE, Data Management Team CLTVT, Epidemiology C, Data Management Team : Tanu Anand HKBPCHCRDAGDAJSKSKVKJPM, *Covid Laboratory Team : Srima Adhikari JANKSGAPAAAAASMAGANAVPA, Virus R, Diagnostic Laboratory Network Team : Arvind Bhushan SGHKJNSRNV. Laboratory surveillance for SARS-CoV-2 in India: Performance of testing & descriptive epidemiology of detected COVID-19, January 22 - April 30, 2020. Indian J Med Res. 2020;151(5):424-37. Epub 2020/07/03. doi: 10.4103/ijmr.IJMR_1896_20. PubMed PMID: 32611914.
  5. Bosch I, Reddy A, de Puig H, Ludert JE, Perdomo-Celis F, Narvaez CF, Versiani A, Fandos D, Nogueira ML, Singla M, Lodha R, Medigeshi GR, Lorenzana I, Ralde HV, Gelvez-Ramirez M, Villar LA, Hiley M, Mendoza L, Salcedo N, Herrera BB, Gehrke L. Serotype-specific detection of dengue viruses in a nonstructural protein 1-based enzyme-linked immunosorbent assay validated with a multi-national cohort. PLoS neglected tropical diseases. 2020;14(6):e0008203. Epub 2020/06/25. doi: 10.1371/journal.pntd.0008203. PubMed PMID: 32579555; PMCID: PMC7351204 (www.e25bio.com), a company that develops point-of-care diagnostics for fever-causing infectious agents.
  6. Arvind B, Medigeshi GR, Kapil A, Xess I, Singh U, Lodha R, Kabra SK. Aetiological agents for pulmonary exacerbations in children with cystic fibrosis: An observational study from a tertiary care centre in northern India. Indian J Med Res. 2020;151(1):65-70. Epub 2020/03/07. doi: 10.4103/ijmr.IJMR_1275_18. PubMed PMID: 32134016; PMCID: PMC7055172.
  7. Kar M, Nisheetha A, Kumar A, Jagtap S, Shinde J, Singla M, M S, Pandit A, Chandele A, Kabra SK, Krishna S, Roy R, Lodha R, Pattabiraman C, Medigeshi GR. Isolation and molecular characterization of dengue virus clinical isolates from pediatric patients in New Delhi. Int J Infect Dis. 2019;84S:S25-S33. Epub 2018/12/12. doi: 10.1016/j.ijid.2018.12.003. PubMed PMID: 30528666; PMCID: PMC6823047.
  8. Kar M, Khan NA, Panwar A, Bais SS, Basak S, Goel R, Sopory S, Medigeshi GR. Zinc Chelation Specifically Inhibits Early Stages of Dengue Virus Replication by Activation of NF-kappaB and Induction of Antiviral Response in Epithelial Cells. Front Immunol. 2019;10(2347):2347. Epub 2019/10/22. doi: 10.3389/fimmu.2019.02347. PubMed PMID: 31632411; PMCID: PMC6779808.
  9. Bais SS, Ratra Y, Khan NA, Pandey R, Kushawaha PK, Tomar S, Medigeshi G, Singh A, Basak S. Chandipura Virus Utilizes the Prosurvival Function of RelA NF-kappaB for Its Propagation. Journal of virology. 2019;93(14). Epub 2019/05/03. doi: 10.1128/JVI.00081-19. PubMed PMID: 31043529; PMCID: PMC6600208.
  10. Medigeshi GR, Fink K, Hegde NR. Position Paper on Road Map for RNA Virus Research in India. Front Microbiol. 2018;9(1753):1753. Epub 2018/08/23. doi: 10.3389/fmicb.2018.01753. PubMed PMID: 30131779; PMCID: PMC6090158.
  11. Ojha A, Nandi D, Batra H, Singhal R, Annarapu GK, Bhattacharyya S, Seth T, Dar L, Medigeshi GR, Vrati S, Vikram NK, Guchhait P. Platelet activation determines the severity of thrombocytopenia in dengue infection. Scientific reports. 2017;7:41697. Epub 2017/02/01. doi: 10.1038/srep41697. PubMed PMID: 28139770; PMCID: PMC5282509.
  12. Kumar R, Singh N, Abdin MZ, Patel AH, Medigeshi GR. Dengue Virus Capsid Interacts with DDX3X-A Potential Mechanism for Suppression of Antiviral Functions in Dengue Infection. Front Cell Infect Microbiol. 2017;7(542):542. Epub 2018/02/02. doi: 10.3389/fcimb.2017.00542. PubMed PMID: 29387631; PMCID: PMC5776122.
  13. Kumar P, Medigeshi GR, Mishra VS, Islam M, Randev S, Mukherjee A, Chaudhry R, Kapil A, Ram Jat K, Lodha R, Kabra SK. Etiology of Acute Respiratory Infections in Infants: A Prospective Birth Cohort Study. Pediatr Infect Dis J. 2017;36(1):25-30. Epub 2016/10/19. doi: 10.1097/INF.0000000000001359. PubMed PMID: 27753796.
  14. Kar M, Singla M, Chandele A, Kabra SK, Lodha R, Medigeshi GR. Dengue Virus Entry and Replication Does Not Lead to Productive Infection in Platelets. Open Forum Infect Dis. 2017;4(2):ofx051. Epub 2017/05/12. doi: 10.1093/ofid/ofx051. PubMed PMID: 28491890; PMCID: PMC5420081.
  15. Jain N, Oswal N, Chawla AS, Agrawal T, Biswas M, Vrati S, Rath S, George A, Bal V, Medigeshi GR. CD8 T cells protect adult naive mice from JEV-induced morbidity via lytic function. PLoS neglected tropical diseases. 2017;11(2):e0005329. Epub 2017/02/06. doi: 10.1371/journal.pntd.0005329. PubMed PMID: 28151989; PMCID: PMC5308832.
  16. Bosch I, de Puig H, Hiley M, Carre-Camps M, Perdomo-Celis F, Narvaez CF, Salgado DM, Senthoor D, O'Grady M, Phillips E, Durbin A, Fandos D, Miyazaki H, Yen CW, Gelvez-Ramirez M, Warke RV, Ribeiro LS, Teixeira MM, Almeida RP, Munoz-Medina JE, Ludert JE, Nogueira ML, Colombo TE, Terzian ACB, Bozza PT, Calheiros AS, Vieira YR, Barbosa-Lima G, Vizzoni A, Cerbino-Neto J, Bozza FA, Souza TML, Trugilho MRO, de Filippis AMB, de Sequeira PC, Marques ETA, Magalhaes T, Diaz FJ, Restrepo BN, Marin K, Mattar S, Olson D, Asturias EJ, Lucera M, Singla M, Medigeshi GR, de Bosch N, Tam J, Gomez-Marquez J, Clavet C, Villar L, Hamad-Schifferli K, Gehrke L. Rapid antigen tests for dengue virus serotypes and Zika virus in patient serum. Sci Transl Med. 2017;9(409). Epub 2017/09/29. doi: 10.1126/scitranslmed.aan1589. PubMed PMID: 28954927; PMCID: PMC6612058.
  17. Singla M, Kar M, Sethi T, Kabra SK, Lodha R, Chandele A, Medigeshi GR. Immune Response to Dengue Virus Infection in Pediatric Patients in New Delhi, India--Association of Viremia, Inflammatory Mediators and Monocytes with Disease Severity. PLoS neglected tropical diseases. 2016;10(3):e0004497. Epub 2016/03/18. doi: 10.1371/journal.pntd.0004497. PubMed PMID: 26982706; PMCID: PMC4794248.
  18. Medigeshi GR, Kumar R, Dhamija E, Agrawal T, Kar M. N-Desmethylclozapine, Fluoxetine, and Salmeterol Inhibit Postentry Stages of the Dengue Virus Life Cycle. Antimicrob Agents Chemother. 2016;60(11):6709-18. Epub 2016/08/31. doi: 10.1128/AAC.01367-16. PubMed PMID: 27572397; PMCID: PMC5075077.
  19. Kumar R, Agrawal T, Khan NA, Nakayama Y, Medigeshi GR. Identification and characterization of the role of c-terminal Src kinase in dengue virus replication. Scientific reports. 2016;6:30490. Epub 2016/07/28. doi: 10.1038/srep30490. PubMed PMID: 27457684; PMCID: PMC4960526.
  20. Kakumani PK, Medigeshi GR, Kaur I, Malhotra P, Mukherjee SK, Bhatnagar RK. Role of human GRP75 in miRNA mediated regulation of dengue virus replication. Gene. 2016;586(1):7-11. Epub 2016/04/04. doi: 10.1016/j.gene.2016.03.053. PubMed PMID: 27039024.
  21. Jenum S, Dhanasekaran S, Lodha R, Mukherjee A, Kumar Saini D, Singh S, Singh V, Medigeshi G, Haks MC, Ottenhoff TH, Doherty TM, Kabra SK, Ritz C, Grewal HM. Approaching a diagnostic point-of-care test for pediatric tuberculosis through evaluation of immune biomarkers across the clinical disease spectrum. Scientific reports. 2016;6:18520. Epub 2016/01/05. doi: 10.1038/srep18520. PubMed PMID: 26725873; PMCID: PMC4698754.
  22. Chandele A, Sewatanon J, Gunisetty S, Singla M, Onlamoon N, Akondy RS, Kissick HT, Nayak K, Reddy ES, Kalam H, Kumar D, Verma A, Panda H, Wang S, Angkasekwinai N, Pattanapanyasat K, Chokephaibulkit K, Medigeshi GR, Lodha R, Kabra S, Ahmed R, Murali-Krishna K. Characterization of Human CD8 T Cell Responses in Dengue Virus-Infected Patients from India. Journal of virology. 2016;90(24):11259-78. Epub 2016/11/01. doi: 10.1128/JVI.01424-16. PubMed PMID: 27707928; PMCID: PMC5126381.
  23. Kakumani PK, Rajgokul KS, Ponia SS, Kaur I, Mahanty S, Medigeshi GR, Banerjea AC, Chopra AP, Malhotra P, Mukherjee SK, Bhatnagar RK. Dengue NS3, an RNAi suppressor, modulates the human miRNA pathways through its interacting partner. The Biochemical journal. 2015;471(1):89-99. Epub 2015/07/30. doi: 10.1042/BJ20150445. PubMed PMID: 26221025.
  24. Kakumani PK, Ponia SS, S RK, Sood V, Chinnappan M, Banerjea AC, Medigeshi GR, Malhotra P, Mukherjee SK, Bhatnagar RK. Role of RNA interference (RNAi) in dengue virus replication and identification of NS4B as an RNAi suppressor. Journal of virology. 2013;87(16):8870-83. Epub 2013/06/07. doi: 10.1128/JVI.02774-12. PubMed PMID: 23741001; PMCID: PMC3754049.
  25. Haridas V, Rajgokul KS, Sadanandan S, Agrawal T, Sharvani V, Gopalakrishna MV, Bijesh MB, Kumawat KL, Basu A, Medigeshi GR. Bispidine-amino acid conjugates act as a novel scaffold for the design of antivirals that block Japanese encephalitis virus replication. PLoS neglected tropical diseases. 2013;7(1):e2005. Epub 2013/01/26. doi: 10.1371/journal.pntd.0002005. PubMed PMID: 23350007; PMCID: PMC3547849.
  26. Agrawal T, Sharvani V, Nair D, Medigeshi GR. Japanese encephalitis virus disrupts cell-cell junctions and affects the epithelial permeability barrier functions. PloS one. 2013;8(7):e69465. Epub 2013/07/31. doi: 10.1371/journal.pone.0069465. PubMed PMID: 23894488; PMCID: PMC3722119.
  27. Agrawal T, Schu P, Medigeshi GR. Adaptor protein complexes-1 and 3 are involved at distinct stages of flavivirus life-cycle. Scientific reports. 2013;3:1813. Epub 2013/05/10. doi: 10.1038/srep01813. PubMed PMID: 23657274; PMCID: PMC3648799.
  28. Medigeshi GR. Mosquito-borne flaviviruses: overview of viral life-cycle and host–virus interactions. Future Virology. 2011;6(9):1075-89. doi: 10.2217/fvl.11.85.
  29. Wertheimer AM, Uhrlaub JL, Hirsch A, Medigeshi G, Sprague J, Legasse A, Wilk J, Wiley CA, Didier P, Tesh RB, Murray KO, Axthelm MK, Wong SW, Nikolich-Zugich J. Immune response to the West Nile virus in aged non-human primates. PloS one. 2010;5(12):e15514. Epub 2010/12/15. doi: 10.1371/journal.pone.0015514. PubMed PMID: 21151986; PMCID: PMC2996299.
  30. Medigeshi GR, Hirsch AJ, Brien JD, Uhrlaub JL, Mason PW, Wiley C, Nikolich-Zugich J, Nelson JA. West nile virus capsid degradation of claudin proteins disrupts epithelial barrier function. Journal of virology. 2009;83(12):6125-34. Epub 2009/04/17. doi: 10.1128/JVI.02617-08. PubMed PMID: 19369347; PMCID: PMC2687390.
  31. Goodman AG, Fornek JL, Medigeshi GR, Perrone LA, Peng X, Dyer MD, Proll SC, Knoblaugh SE, Carter VS, Korth MJ, Nelson JA, Tumpey TM, Katze MG. P58(IPK): a novel "CIHD" member of the host innate defense response against pathogenic virus infection. PLoS pathogens. 2009;5(5):e1000438. Epub 2009/05/23. doi: 10.1371/journal.ppat.1000438. PubMed PMID: 19461876; PMCID: PMC2677460.
  32. Medigeshi GR, Krikunova M, Radhakrishnan K, Wenzel D, Klingauf J, Schu P. AP-1 membrane-cytoplasm recycling regulated by mu1A-adaptin. Traffic. 2008;9(1):121-32. Epub 2007/11/09. doi: 10.1111/j.1600-0854.2007.00672.x. PubMed PMID: 17988225.
  33. Medigeshi GR, Hirsch AJ, Streblow DN, Nikolich-Zugich J, Nelson JA. West Nile virus entry requires cholesterol-rich membrane microdomains and is independent of alphavbeta3 integrin. Journal of virology. 2008;82(11):5212-9. Epub 2008/04/04. doi: 10.1128/JVI.00008-08. PubMed PMID: 18385233; PMCID: PMC2395215.
  34. Medigeshi GR, Lancaster AM, Hirsch AJ, Briese T, Lipkin WI, Defilippis V, Fruh K, Mason PW, Nikolich-Zugich J, Nelson JA. West Nile virus infection activates the unfolded protein response, leading to CHOP induction and apoptosis. Journal of virology. 2007;81(20):10849-60. Epub 2007/08/10. doi: 10.1128/JVI.01151-07. PubMed PMID: 17686866; PMCID: PMC2045561.
  35. Scott GK, Fei H, Thomas L, Medigeshi GR, Thomas G. A PACS-1, GGA3 and CK2 complex regulates CI-MPR trafficking. The EMBO journal. 2006;25(19):4423-35. Epub 2006/09/16. doi: 10.1038/sj.emboj.7601336. PubMed PMID: 16977309; PMCID: PMC1589982.
  36. Hirsch AJ, Medigeshi GR, Meyers HL, DeFilippis V, Fruh K, Briese T, Lipkin WI, Nelson JA. The Src family kinase c-Yes is required for maturation of West Nile virus particles. Journal of virology. 2005;79(18):11943-51. Epub 2005/09/06. doi: 10.1128/JVI.79.18.11943-11951.2005. PubMed PMID: 16140770; PMCID: PMC1212629.
  37. Medigeshi GR, Schu P. Characterization of the in vitro retrograde transport of MPR46. Traffic. 2003;4(11):802-11. Epub 2003/11/18. doi: 10.1034/j.1600-0854.2003.00136.x. PubMed PMID: 14617361.
  38. Roy N, Guruprasad MR, Kondaiah P, Mann EA, Giannella RA, Visweswariah SS. Protein kinase C regulates transcription of the human guanylate cyclase C gene. European journal of biochemistry / FEBS. 2001;268(7):2160-71. Epub 2001/03/30. doi: 10.1046/j.1432-1327.2001.02101.x. PubMed PMID: 11277940.
  39. Meyer C, Eskelinen EL, Guruprasad MR, von Figura K, Schu P. Mu 1A deficiency induces a profound increase in MPR300/IGF-II receptor internalization rate. Journal of cell science. 2001;114(Pt 24):4469-76. Epub 2002/01/17. PubMed PMID: 11792812.
  40. Vijayachandra K, Guruprasad M, Bhandari R, Manjunath UH, Somesh BP, Srinivasan N, Suguna K, Visweswariah SS. Biochemical characterization of the intracellular domain of the human guanylyl cyclase C receptor provides evidence for a catalytically active homotrimer. Biochemistry. 2000;39(51):16075-83. Epub 2000/12/22. doi: 10.1021/bi0013849. PubMed PMID: 11123935.
  • Charles Grose, L. M., Guruprasad R. Medigeshi, Gregory K. Scott, and Gary Thomas. (2006) Endocytosis of Varicella Zoster Virus Glycoproteins: Virion Envelopment and Egress, Horizon Scientific Press

 

S. NO.
Publication ‘title’ and date of publication Patents filed ‘title’ and date fo filing Patents granted ‘title’ and date Remarks, if any
1

Zinc Chelation Specifically InhibitsEarly Stages of Dengue Virus

Replication by Activation of NF-kB

and Induction of Antiviral Response

in Epithelial Cells (Frontiers in Immunology; 01-October-2019)

Zinc chelator as inhibitor of dengue virus replication and uses thereof

04th Feb, 2019

Application no. TEMP/E1/13889/2019-DEL  
2 In process A DNAzyme-based colorimetric detection of SARS-CoV-2 and its uses thereof TEMP/E-1/22576/2020-DEL  

 

  • Ph.D. fellowship from Boehringer Ingelheim Foundation, Germany.
  • Wellcome-DBT Intermediate Fellowship - 2014 - 2020.

 

Aleksha Panwar

PhD Student – 2016 - till date
M.Sc in Microbiology (2015) - CCS University, Meerut in 2015.
Following post-graduation I worked as a volunteer in a NGO. After that, I decided to extend my education.
aleksha@thsti.res.in

My current project focuses on understanding the role of innate immunity and host factors in dengue pathogenesis. Outside of my academic interests, I enjoy listening to music, playing badminton, travelling and reading novels.

 


Amresh Kumar Singh

Lab Technician – 2011 – Present
B.A. (Hons) in History, Bihar University

I have been working in research labs since 1998 and play an important role in keeping our lab functional and productive. I take care of all day-to-day research requirements of the lab.

 

 


Sunil

Project Associate - I
M.Sc. in Botany (2018) from Maharshi Dayanand University, Rohtak, Haryana.

I was teaching ecology, evolution and genetics at GGDSD College Palwal. This is my first employment in a research laboratory.

I play cricket and like to read novels.

 

 


Dr. Jigme Wangchuk

jigmewangchuk@thsti.res.in
Research Associate since November, 2019

M.Sc.-Ph.D. integrated from Indian Institute of Technology, Bombay
B.Sc. Biotechnology from Indian Academy Degree College, Bangalore University

My doctoral research work focused on understanding the mechanism of genome packaging in bacteriophage N4. The study enabled us to understand how bacteriophages evolves and maintain their assembly fidelity. I am currently working on characterizing the role of zinc homeostasis in RNA virus life-cycle.

I enjoy reading football blogs and listening to podcasts on science, sports and music.


Dr. Saurabh Kumar

saurabh@thsti.res.in
Junior Research Scientist

M.Sc. in Molecular Biology and Biotechnology, Tezpur University, Assam
Ph.D. from Indian Institute of Science, Bangalore

During my doctoral research I worked on the Molecular basis of attenuation of the live attenuated Japanese encephalitis virus vaccine SA14-14-2. I am currently working on isolation and characterization of the Dengue and SARS-CoV-2 viruses prevalent in India.

I like solving puzzle and playing volleyball.

 


Moushumee Das Verma

 

moushumee@thsti.res.in
Junior Research Scientist since November 2020

Ph. D. from Indian Institute of Technology Guwahati, Assam
M. Sc. Molecular Biology and Biotechnology, Tezpur University, Assam

B. Sc. Biotechnology, North Eastern Hill University, Meghalaya

My doctoral dissertation focused on understanding Newcastle Disease Virus (paramyxovirus) evolution in Northeast India and development of effective diagnostics and antiviral therapeutics. I am currently working on development of prophylactic and therapeutic strategies for Dengue in Indian subcontinent.

I am an avid learner and thrive to learn and keep myself dynamic, visionary and competitive with the changing scenario of the world. I enjoy music, art, travel and photography.


Monika Bharadwaj

Project Associate - II

CSIR-CDRI Lucknow as a Research fellow till Jan 2020
M.Sc in Biotechnology from Maharshi Dayanand University Rohtak, Haryana
Graduation in biotechnology from KL Mehta Dayanand college for women's Faridabad

I enjoy playing badminton and exploring new things.

 


 

Former students:

 

Rinki Kumar (2012 – 2018)

Currently working as Post-doctoral fellow
Penn State Health
Milton S. Hershey Medical Center, Hershey, PA 17033, USA
 

 

 

 


Meenakshi Kar (2013-2019)

Current Position: Grants Advisor, India Alliance