Screen Reader Access   

People : Scientific : Faculty & Scientists

DR. TARUN KUMAR SHARMA, Ph.D  

Senior Research Scientist
+91-129-2876370 
tarun [at] thsti [dot] res [dot] in
Ph.D


Areas of Expertise

  • In vitro SELEX and Aptamer Technology
  • Affordable diagnostics
  • Electrochemical sensors and
  • Nanomaterials based Biosensors

 

Research Interests/ Research Summary

Our group is primarily focusing on

  • Development of Aptamer based rapid diagnostic test for infectious disease, pollutants and toxins.
  • Application of nanomaterials for diagnostics and therapeutics. 
  • Aptamer based therapeutics 

 For complete list of publication please visit Google Scholar Profile https://scholar.google.co.in/citations?user=7JdrxHsAAAAJ&hl=en

* Represent publication as corresponding author

 Journal publications (*Indicate publication as corresponding author)

2021

Singhal, C.; Bruno, J. G.; Kaushal, A.; Sharma, T. K *.  Recent Advances and a Roadmap to Aptamer-Based Sensors for Bloodstream Infections. ACS Applied Biomaterials (2020), 593, 113574. *as corresponding author, In press (https://dx.doi.org/10.1021/acsabm.0c01358)

 

2020

1. Reid, R.; Chatterjee, B.; Jyotidas, S.; Ghosh, S.; Sharma, T. K *.  Application of aptamers as molecular recognition elements in lateral flow assays. Analytical Biochemistry (2020), 593, 113574. *as corresponding author, IF-2.87

 2. Chatterjee, B.; Das S. J.; Anand, A.; Sharma, T. K *.  Nanozymes and aptamer-based biosensing. Materials Science for Energy Technologies (2020), 3, 127-135. *as corresponding author, Invited article for special issue on Sensors

3. Chatterjee, B.; Das S. J.; Anand, A.; Sharma, T. K *.  Aptamer-NanoZyme mediated sensing platform for the rapid detection of Escherichia coli in fruit juice. Sensing and Biosensing Research (2020), 27, 100313. *as corresponding author. Citescore 5.9

4. Shankar, U.; Jain, N.; Mishra S. K.; Sharma, T. K.; Kumar A.  Conserved G-Quadruplex Motifs in Gene Promoter Region Reveals a Novel Therapeutic Approach to Target Multi-Drug Resistance Klebsiella pneumoniae. Frontiers in microbiology (2020) 11, 1269. IF-4.23.

5. Jain, N.; Mishra S. K.; Shankar, U.; Jaiswal, A.; Sharma, T. K.; Kodgire, P.; Kumar A.  G-quadruplex stabilization in the ions and maltose transporters gene inhibit Salmonella enterica growth and virulence. Genomics (2020) 112, 4863-4874. IF-6.2.

6. Chatterjee, B.; Kalyani, N.; Anand, A.; Khan, E.; Das S. J.; Bansal, V.; Kumar, A.; Sharma, T. K.* GOLD SELEX: A novel SELEX approach for the development of high-affinity aptamers against small molecules without residual activity. Microchimica Acta (2020) 186, 618. *as corresponding author IF-6.23

7. Taneja, V.; Goel, M.; Shankar, U.; Kumar, A.; Khilani  G. C.; Prasad, H. K.; Prasad, GBKS..;  Gupta, U. D.; Sharma, T. K.* An Aptamer Linked Immobilized Sorbent Assay (ALISA) to detect circulatory IFN-α, an inflammatory protein among Tuberculosis patients. ACS Combinatorial Science(2020
) https://doi.org/10.1021/acscombsci.0c00108(Ahead of print) *as corresponding author IF-3.3

 

8. Anantharaj, A.; Das, S. J.; Patil, S.; Lodha, R.; Kabra  S. K.; Sharma, T. K.*, Medigeshi, G. M.* Visual Detection of SARS-CoV-2 RNA by Conventional PCR-Induced Generation of DNAzyme Sensor. Frontiers in molecular Biosciences  (2020), 7, :586254. doi: 10.3389/fmolb.2020.586254

*as corresponding author IF-4.5

 

2019

1.Dhiman, A.; Kumar, C.; Mishra, S.K.; Sikri, K.; Haldar S.; Sharma, N.; Bansal, A.; Ahmad., Y.; Kumar. A.; Sharma, T. K *, Tyagi J. S*Theranostic application of a novel G-Quadruplex forming DNA aptamer targeting glyoxylate cycle enzyme malate synthase of Mycobacterium tuberculosis. Molecular therapy Nucleic Acids-(Cell Press). (2019), 18. 661-672.IF-7.0 * as corresponding author

2. Mishra, S. K.; Jain, N.; Shankar, U.; Tawani, A.; Sikri, K., Tyagi, J.S., Sharma, T. K, Margny, J.L., Kumar A.; Characterization of G-quadruplex motifs in espB, espK and cyp51 genes of Mycobacterium tuberculosisas a potential drug target. Molecular therapy Nucleic Acids-(Cell Press).(2019), 16. 698-706. IF-7.0

3. Das, R.; Dhiman, A.; Mishra, S. K.; Haldar S.; Sharma, N.; Bansal, A.; Ahmad., Y.; Kumar. A.; Tyagi J. S, Sharma, T. K *,.  Structural switching electrochemical DNA aptasensor for the rapid diagnosis of tuberculous meningitis. International Journal of Nanomedicine.(2019), 14.2103-2113.

* as corresponding author IF-5.0

4. Das, R.; Dhiman, A.; Kapil, A.; Bansal V.; Sharma, T. K *.  Aptamer-mediated colorimetric and electrochemical detection of Pseudomonas aeruginosa utilizing peroxidase-mimic activity of gold NanoZyme.Analytical Bioanalytical Chemistry.(2019), 411.1229-1238.

* as corresponding authorIF-3.63Featured on Chembites (A blog created and maintained by young American scientists)

    https://chembites.org/2019/03/26/fishing-for-bacteria-with-gold-and-dna/

5. Kumari, P.; Lavania, S.; Tyagi, S.; Dhiman, A.; Rath, D.; Anthwal, D.; Gupta, R. K.; Sharma, N. Gadpayle, A. K.; Taneja, R. S.; Sharma, N.; Sharma, T. K*, Haldar, H.; Tyagi J. S.*; A novel aptamer based test for the rapid and accurate diagnosis of pleural tuberculosis    Analytical Biochemistry(2019)1. 564-565.* as corresponding Author IF-2.87

6. Mishra, S. K.; Jain, N.; Shankar, U.; Tawani, A.; Sharma, T. K, Kumar A.; Characterization of highly conserved G-quadruplex motifs as potential drug targets in Streptococcus pneumoniae. Scientific Reports (2019) 9. 1-13.IF-4.0

2018

1.Kalra, P,Mishra S K.; Kaur S.; Prasad, H, P.; Kumar, A.; Sharma, T. K *.; Tyagi J. S*.  G-quadruplex   forming DNA aptamers inhibit DNA-binding function of HupB (Rv2986c) and Mycobacterium tuberculosis entry into host cells. Molecular Therapy Nucleic Acids (Cell Press).(2018) 13, 99-109.* as corresponding author IF-7.0

Media coverage: This article was highlighted in ‘The Hindu’ https://www.thehindu.com/sci-tech/science/aptamer-inhibits-tb-bacteria-entry-into-cells/article24903152.ece

2. Dhiman, A.; Haldar S.; Mishra, S.K.; Sharma, N.; Bansal, A.; Ahmad., Y.; Kumar. A.; Sharma, T. K *, Tyagi J. S*Generation and application of DNA aptamers against HspX for accurate diagnosis of tuberculous meningitis. Tuberculosis(2018) 112, 27–36. * as corresponding author IF-2.8  Media coverage: This article was highlighted in ‘The Hindu’ https://www.thehindu.com/sci-tech/science/aiims-led-team-develops-highly-sensitive-portable-test-for-tb-meningitis/article24541516.ece

3. Dhiman, A.; Kalra, P,Dhiman A.; Bruno J.G.; Sharma, T. K *.  Simple Methods and Rational Design for Enhancing Aptamer Sensitivity and Specificity. Frontiers in Molecular Bioscience(2018) 5:41.doi: 10.3389/fmolb.2018.00041. * as corresponding author IF-4.18

4. Kaur H.; Bruno J.G.; Sharma, T. K *.  Aptamers in the Therapeutics and Diagnostics Pipelines. Theranostics(2018) 8, 4016-4032. * as corresponding author, IF-8.0

5. Dhiman, A.; Anand, A.; Malhotra, A.; Khan, E.; Santra, V.; Kumar, A.; Sharma, T. K *. Rational truncation of aptamer for cross-species application to detect krait envenomation. Scientific Reports.(2018) 12, 17795.* as corresponding author, IF-4.0

Media coverage:A step closer to detecting krait venom’ https://www.thehindu.com/sci-tech/science/a-step-closer-to-detecting-krait-venom/article25807488.ece

https://www.natureasia.com/en/nindia/article/10.1038/nindia.2019.2

6. Lavania, S.; Dhiman, A.; Myneedu, V.P.; Verma, A.; Singh,N.; Sharma, T. K*, Tyagi J. S.*;Aptamer-  Based TB Antigen Tests for the Rapid Diagnosis of Pulmonary Tuberculosis: Potential Utility in Screening  for Tuberculosis. ACS Infectious Disease(2018) 4, 1718–1726. * as corresponding author IF-4.61

Media coverage: This article was highlighted in ‘Science Chronicle’   https://journosdiary.com/2018/11/12/podcast-diagnostic-tests-tb/

7. Abraham A, Sharma T. K.; Shukla R.; Bansal V. Phytochemicals as dynamic surface ligands to control nanoparticle–protein interactions. ACS Omega(2018) 3, 2220-2229. IF-2.8

8. Kaur H.; Kumar A.; Sharma, T. K .  Analytical techniques for characterization of biological molecules/  proteins and aptamers/oligonucleotides. Bioanalysis(2019), 11, 103-117. IF-2.37

2017

1.  Sharma, T. K.*; Bruno J.G.; Dhiman, A. ABC of DNA aptamers and related assay developments. Biotechnology Advances(2017), 35 275–301. * as corresponding author IF-10.74

2.     Dhiman A.; Kalra P.;Bruno J.G.; Sharma, T. K*. Aptamer based point-of-care diagnostic platforms Sensors and Actuators B:Chemical(2017), 246,  535–553. * as corresponding author IF-7.1

2016

1.  Sharma, T. K.*; Bruno J.G.; Cho W. The point behind translation of aptamers for point-of-care diagnostics.Aptamers and Synthetic Antibodies,(2016) 2(2): 36-42.

2. Gupta M.; Mathur, S.; Sharma, T. K.; Rana M.; Navani N.K.; Pathania, R.  A study on metabolic prowess of Pseudomonas sp. RPT 52 to degrade imidacloprid, endosulfan and coragen. Journal of Hazardous Material(2016), 30, 250-258. IF-9.0

3. Dhillon A.; Sharma, T. K.; Soni, S. M.; Kumar.D.; Fluoride adsorption on a cubical ceria nanoadsorbent: function of surface properties. RSC Advances(2016), 6, 89198-89209. IF-3.11

2015

1.       Sahkir, M.; Jolly, R.; Khan, M. S.; Iram, N.; Sharma, T. K.; Alresayes, S. I. Synthesis and characterization of a nano-hydroxyapatite/chitosan/polyethylene glycol nanocomposite for bone tissue engineering. Polymer for Advanced Technologies, (2015), 26, 41-48DOI: 10.1002/pat.3417.IF-2.57

2.     Lambadi, P. R.; Sharma, T. K.$; Kumar P.; Vasnani, P.; Mouli, S. R.; Bisht N.; Pathania, R.; Navani, N. K. Facile bio-functionalization of silver nanoparticles for enhanced antibacterial properties, endotoxin removal and biofilm control. International Journal of Nanomedicine. (2015),10, 2155-2177. Joint First Author IF-5.0

3.       Sharma, T. K.; Ramanathan. R.; Rakwal, R.; Agrawal, G. K.; Bansal, V. Moving forward in plant food safety and security through NanoBioSensors: Adopt or adapt biomedical technologies? Proteomics (2015), 10, 1680-1692. IF-3.1

2014

1.    *Sharma, T. K.; Ramanathan. R.;.; Mohammadtaheri, M.; Daima, H. K.; Shukla, R.; Bansal, V. Aptamer-mediated ‘turn-off/turn-on’ nanozyme activity of gold nanoparticles for kanamycin detection. Chemical Communications. (2014), 50, 15856-15859.featured on Cover Page.  * as corresponding author IF-5.99

2.       Weerathunge, P Ramanathan. R.; Shukla, R.; *Sharma, T. K.; Bansal, V. Aptamer-controlled reversible inhibition of gold nanozyme activity for pesticide sensing. Analytical Chemistry (2014), 86,11937–11941. featured on Cover Page .* as corresponding author IF-6.78

3.       Chopra, A.; Shukla, R.; *Sharma, T. K. Aptamers as an emerging player in Biology. Aptamers and Synthetic Antibodies (2014), 1, 1-11. Inaugural issue. * as corresponding author

4.       *Sharma, T. K.; Shukla, R. Nucleic acid aptamers as an emerging diagnostic tool for animal pathogens. Advances in Animal and Veterinary Sciences2014, 2(1) 50-55. * as corresponding author

5.       *Sharma, T. K.; Shukla, R. Editorial Nanoscience and Aptamer Technology for Point of Care Diagnostics. Nanoscience and Technology2014, 1(2) 1-2. Editorial

2013

1.       Ghosh, I. N.; Patil , S.D. Sharma, T. K.; Shrivastava, S. K.; Pathania, R.; Navani, N. K. Synergistic action of cinnamaldehyde with silver nanoparticles against spore-forming bacteria: a case for judicious use of silver nanoparticles for antibacterial applications. International Journal of Nanomedicine (2013), 8: 4721 IF-5.0

2012

1.       Lange, M. J.; Sharma, T. K.; Whatley, A. S.; Landon, L. A.; Tempesta, M. A.; Johnson M. C.; Burke, D. H.  Robust suppression of HIV replication by intracellularly expressed reverse transcriptase aptamers is independent of ribozyme processing. Molecular Therapy (Cell Publishing Group) (2012), 20: 2304-2314. IF-8.4

2.       Sharma, T. K.;Sapra M.; Chopra A.; Sharma R.; Malik, M.; Pathania, R.; Navani, N. K.; Interaction of bacteriocin-capped silver nanoparticles with food pathogens and their antibacterial effect. International Journal of Green Nanotechnology(2012), 4(2), 93-110.

3.       Sharma, T. K.;Chopra A.; Sapra M.; Kumanwat D.; Pathania, R.; Navani, N. K.; Green synthesis and antimicrobial potential of silver nanoparticles. International Journal of Green Nanotechnology2012, 4(1), 1-16.

 

 

1.       Kaur, H.; Chaterjee, B.; Bruno, J. G..; Sharma, T. K. Defining Target Product Profiles (TPPs) for aptamer-based diagnostics, Adv Biochem Eng Biotechnol. 2019 Jul 23. doi: 10.1007/10_2019_104. [Epub ahead of print]

2.       Chaterjee, B.; Kalyyani, N.; ; Jyotidas. S.; Anand, A. Sharma, T. K. Nano-realm for point-of-care (POC) bacterial diagnostics, Chapter:2, Methods in Microbiology 46 (2019) 19-37 (Elsevier Academic Press)-2019

3.       Dhiman, A.; Kaur, H.; Kumar, C.; Ahmad. Y.; Sharma, T. K. Applications of Aptasensors in Health Care,  Chapter:1, Biosensors: Materials and Applications,  Materials Research Foundations 47 (2019) 1-50 (Materials Research Forum LLC)-2019

4.       Weerathunge, P.; Sharma, T. K.; Ramanathan, R.; Bansal, V.;Nanozyme-based environmental Monitoring. Advanced Environmental Analysis: Applications of Nanomaterials, Volume 2 Chapter: 23 (Royal Society of Chemistry  UK)-2016

5.   Chopra, A.Sharma, T. K.; Pathania,  R.; Navani, N. K. Development of an aptamer- nanoparticles based nanobiosensor for bacterial detection. Sinha, S., Navani, N. K. (Eds.), Studium Press-USA, 2013, Volume 10, Chapter 4,  (ISBN: 1-62699-000-10).

6.       Sapra, M.Sharma, T. K.; Pathania,  R.; Navani, N. K.  Nanoparticles based optical detection of xenobiotics and small molecules. Sinha, S., Navani, N. K. (Eds.), Studium Press-USA, 2013, Volume 10, Chapter 8,  (ISBN: 1-62699-000-10).

7.    *Sharma, T. K.; Sharma, K. K.  Pesticide in soil environment and their microbial degradation. Bharti, P. K., Zaki, M. S. (Eds.), Discovery publishing house-India, 2013, Chapter 6, (ISBN: 9-5056-313-4).

Book chapters

 

1.       Kaur, H.; Chaterjee, B.; Bruno, J. G..; Sharma, T. K. Defining Target Product Profiles (TPPs) for aptamer-based diagnostics, Adv Biochem Eng Biotechnol. 2019 Jul 23. doi: 10.1007/10_2019_104. [Epub ahead of print]

 

2.       Chaterjee, B.; Kalyyani, N.; ; Jyotidas. S.; Anand, A. Sharma, T. K. Nano-realm for point-of-care (POC) bacterial diagnostics, Chapter:2, Methods in Microbiology 46 (2019) 19-37 (Elsevier Academic Press)-2019

 

3.       Dhiman, A.; Kaur, H.; Kumar, C.; Ahmad. Y.; Sharma, T. K. Applications of Aptasensors in Health Care,  Chapter:1, Biosensors: Materials and Applications,  Materials Research Foundations 47 (2019) 1-50 (Materials Research Forum LLC)-2019

4.       Weerathunge, P.; Sharma, T. K.; Ramanathan, R.; Bansal, V.;Nanozyme-based environmental Monitoring. Advanced Environmental Analysis: Applications of Nanomaterials, Volume 2 Chapter: 23 (Royal Society of Chemistry  UK)-2016

 

5.   Chopra, A.Sharma, T. K.; Pathania,  R.; Navani, N. K. Development of an aptamer- nanoparticles based nanobiosensor for bacterial detection. Sinha, S., Navani, N. K. (Eds.), Studium Press-USA, 2013, Volume 10, Chapter 4,  (ISBN: 1-62699-000-10).

6.       Sapra, M.Sharma, T. K.; Pathania,  R.; Navani, N. K.  Nanoparticles based optical detection of xenobiotics and small molecules. Sinha, S., Navani, N. K. (Eds.), Studium Press-USA, 2013, Volume 10, Chapter 8,  (ISBN: 1-62699-000-10).

7.    *Sharma, T. K.; Sharma, K. K.  Pesticide in soil environment and their microbial degradation. Bharti, P. K., Zaki, M. S. (Eds.), Discovery publishing house-India, 2013, Chapter 6, (ISBN: 9-5056-313-4).

1. Novel DNA aptamers against nucleocapsid protein of SARS-CoV-2 and uses thereof. Patent Number. 202011034641

2.A DNAzyme-based colorimetric detection of SARS-Cov-2 and Its uses thereof. Patent Number. 202011020560

3. Novel DNA aptamers against spike protein of SARS-CoV-2 and uses thereof. Patent Number. 202011017852

4. High-affinity aptamers against dichlorvos and a process for producing the same. Patent Number 202011004692

5. Novel DNA aptamers against venom of Bungarus caeruleus (Common Indian Krait), method of its chemical synthesis and uses thereof. Patent Number 201911036350

6. Aptamers against Phospholipase A2 in snake venom and uses thereof. Patent Number 201911027455

7. Aptamer based electrochemical Biosensor for the rapid diagnosis of tuberculous meningitis.Patent Number 201811042593 and PCT/IN20 19/050827 Publication date 22nd May 2020.-Technology transferred to Mol Bio Diagnostic Pvt. Ltd.

8. Aptamer against M.tb MPT51 and uses thereof.Patent Number PCT/IN2018/050581 and US2020/0283774A1) Publication date 10th Sep 2020.

9. Aptamer against M.tb MPT51 and uses thereof.Patent application Number 201811023360

10.Novel DNA aptamers against nucleoid-associated protein HupB of Mycobacterium tuberculosis and uses thereof.Patent Number 201711001246

11. Aptamer against mycobacterial malate synthase and uses thereof. Patent Number 201611021901

12. Novel DNA aptamers against heat shock protein HspX of mycobacterium tuberculosis and uses thereof. Patent Number 201611001550 Technology transferred to Mol Bio Diagnostic Pvt. Ltd.

 

 

Recently approved grants

1. Title of grant: An aptamer-based approach to expedite accurate diagnosis of bacterial and fungal sepsis in neonates

Role: PI 

Duration: 5.0 years (2021-2026)

Funding Agency: DBT

Amount: Rs. 165.00 lakhs

 

2. Title of grant: Mission-Next Generation Treatment for Snakebite (NGTS)

Role:PI 

Duration: 5.0 years (2021-2026)

Funding Agency: DBT

Amount: Rs. 92.84 lakhs

 

 

Ongoing Research Projects

1. Title of grant: Development of aptamer-based assay for the diagnosis of breast tuberculosis (Breast TB).  as PI 

Duration: 2.0 years (2019-2021)

Funding Agency: AIIMS-THSTI

Amount: Rs. 20.00 lakhs (total grant); Sanctioned to THSTI 10.0 Lakhs.

 

2. Title of grant: Aptamer based rapid test to differentiate venomous snake bites from non-venomous and dry snake bites. - as PI

Duration: 1.5 years (2018-2019)

Funding Agency: BIRAC under PACE scheme

Amount: Rs. 48.65 lakhs (total grant); Sanctioned to THSTI 36.40 Lakhs.

 

3. Title of grant:  Aptamer-based Tuberculosis Diagnostics Toolbox - as PI

Duration: 3.0 years (2018-2020)

Funding Agency: DBT under call for TB diagnostics

Amount: Rs. 65 lakhs (total grant) Sanctioned to THSTI 35 Lakhs.

 

 2020                       2nd prize in IKMC-2020 Future Scan conference in MedTech categoryorganized by  

                                   IKP, USP and DUPONT.

 2020                       Mentor for RMIT Australia-Technology and Entrepreneurship Club.

 2020                       Reviewer for BIRAC-SITARE-Scheme

 2019                       Indian National Science Academy (INSA) Young Scientist Medal

 2019                      Reviewer for Gandhian Young Technological Innovation (GYTI) Award  

 2018                      Winner of technology showcasing competition at IKMC-2018 organized by IKP and

                                   DUPONT

2018                      Member of DBT-Task Force for grant evaluation & making decision on funding

2018                      Nominated as a Future Science Leader by DBT-Secretary and STS-Japan

2018                      Selected as a Mentor for “Aavishkar, the innovation club” of AIIMS-Patna

2018                      IGNITE Fellow-2018 at Judge Business School Cambridge University-UK

2017                      DBT Innovative Young Biotechnologist Award (IYBA)

2014                     Australian Endeavour Research Award (Commonwealth of Australia)

2014                     Indo-Australian Career Boosting Research Fellowship (DBT-India and Australian Research Council)-Not Availed

2014                     Indo-Finnish Diagnostic Fellowship (DBT-India and Government of Finland) -Not Availed

2012                    Innovation Award, Department of Biotechnology- Govt. of India

2010                    Visiting Research Fellowship- National Institute of Health- USA in Prof. Donald H. Burke’s group

2009                    National Doctoral Fellowship (AICTE-India)

2007                    National Eligibility Test (Eligibility for Lectureship) cum Junior Research Fellowship


MS. ANJALI ANAND
TECHNICAL OFFICER-I

MR. SOON JYOTI DAS
PROJECT ASSOCIATE-II

Dr. Chaitali Shinghal 

Mr. Abhijeet Dhiman

Ms. Anjali Anand

Mr. Ankit Gupta

Mr. Akshay Jain