Screen Reader Access   

People : Scientific : Faculty & Scientists

DR. AJAY KUMAR, Ph.D. Life Sciences, Guru Nanak Dev University, Amritsar  

Senior Research Scientist
+91-129-2876488 
a.kumar [at] thsti [dot] res [dot] in
M.Sc. Molecular Biology and Biochemistry, Guru Nanak Dev University, Amritsar
B.Sc. Biology, Guru Nanak Dev University, Amritsar


  1. Broad interest of my research includes identifying pathways or proteins involved in early stages of progression of metabolic diseases including Type 2 diabetes, obesity, etc. I and my team members are working closely with the mathematics team at DDRC to identify drug targets from high throughput proteomic and metabolomic datasets. Our focus is to study context and time specific changes in the proteome and metabolome of different tissue types from diseased animal models compared to their normal counterparts. The putative drug targets are validated by employing different molecular approaches including CRISPR-Cas9 based genome editing, siRNA known-downs, protein over-expression employing lentivirus based or gateway cloning strategies, etc. We are specifically focused on developing in-house expertise on designing CRISPR-Cas9 based genome editing tools and exploring their diverse applications in drug target discovery. 

 

Previous Jobs/Trainings

  • Scientist B at Immunology group, ICGEB, New Delhi (2011-2016)
  • Research Scientist at Immunology Group, ICGEB, New Delhi (2010-2011)

 

2020

  1. Effect of delay in transportation of extracellular glucose into cardiomyocytes under diabetic condition: A study through mathematical model. Journal of Biological Physics. (https://doi.org/10.1007/s10867-020-09551-8)

  2. c.29C>T polymorphism in the TGF-β1 gene correlates with increased risk of End Stage Renal Disease: original study and meta-analysis. Meta Gene (https://doi.org/10.1016/j.mgene.2020.100703)

  3. HyperQuant – a computational pipeline for higher order multiplexed quantitative proteomics. ACS Omega (https://doi.org/10.1021/acsomega.0c00515)

2019

  1. Outlining the Grb2 Interactome and its Interacting Partners in HEK293 cells in Absence and Presence of Epidermal Growth Factor. Data in Brief (https://doi.org/10.1016/j.dib.2019.104082)

  2. Protective effect of high levels of TGF-β1 cytokine and its corresponding allele at C-509T locus in Punjabi ESRD population. Meta Gene (https://doi.org/10.1016/j.mgene.2019.100587)

2018

  1. Rb interactome data and its modulations during cell cycle progression in HEK 293 cells. Data in Brief (https://doi.org/10.1016/j.dib.2018.01.045)

  2. Concurrent interactome and metabolome analysis reveals role of AKT1 in central carbon metabolism. BMC Research Notes (https://doi.org/10.1186/s13104-018-3364-z)

  3. Defining the Akt1 interactome and its role in regulating the cell cycle. Scientific Reports (https://doi.org/10.1038/s41598-018-19689-0)

2017

  1. Restoring calcium homeostasis in diabetic cardiomyocytes: An investigation through mathematical modelling. Molecular BioSystems (https://doi.org/10.1039/c7mb00264e)

  2. Dataset to delineate changes in association between Akt1 and its interacting partners as a function of active state of Akt1 protein. Data in Brief (https://doi.org/10.1016/j.dib.2017.05.040)

  3. Defining the Akt1 Interactome Data and Delineating Alterations in its Composition as a function of Cell Cycle Progression. Data in Brief (https://doi.org/10.1016/j.dib.2017.02.018)

2016

  1. Dataset generated using hyperplexing and click chemistry to monitor temporal dynamics of newly synthesized macrophage secretome post infection by mycobacterial strains. Data in Brief (https://doi.org/10.1016/j.dib.2016.08.055)

2015

  1. M235T polymorphism in the AGT gene and A/GI8-83 substitution in the REN gene correlate with end stage renal disease. Nephron (https://doi.org/10.1159/000370074)

2014

  1. Association of tumor necrosis factor-alpha -308 G>A polymorphism with rheumatoid arthritis in two north Indian cohorts. Archives of Rheumatology 29: 241- 249

  2. Ajay Kumar, Vikas Gupta, Manoj Chaudhary, Arvinder Singh, Prabodh K Sehajpal (2014) Depleted TGF-β1 levels in end stage renal disease patients from North India. Gene 534: 440-443

2012

  1. Genomic Diversity and Affinities in population groups of North West India: An analysis of Alu insertion and a single nucleotide polymorphism. Gene 511: 293–299.

2008

  1. Tumor Necrosis Factor-α and Transforming growth factor-β1 polymorphisms in Bronchial Asthma. Indian Journal of Medical Sciences 62: 323-330.

2007

  1. Transforming Growth factor-βC-509T promoter polymorphism in End Stage Renal Disease. ICFAI Journal of Biotechnology 1: 29-36.

  2. Transforming Growth Factor-beta1 gene polymorphism in tuberculosis patients. International Journal of Human Genetics 7: 249-252

2006

  1. Association study of Angiotensin-Converting Enzyme Gene Ins/Del polymorphism with hypertension in Punjabi population. International Journal of Human Genetics 6: 317- 321

  2. Effect of Drug therapy on transforming growth factor-βlevels in end stage renal disease patients. Journal of Punjab Academy of Sciences 3: 1-6

  3. Angiotensin converting enzyme gene Insertion/Deletion polymorphism in Type 2 Diabetic (T2DM) patients from Punjab. Journal of Punjab Academy of Sciences 3: 7-12

2005

  1. Angiotensin-I converting enzyme I/D polymorphism and Diabetic nephropathy in North India. International Journal of Human Genetics 5: 279-283

  • A Herbal Composition from Premna herbacea, useful for prevention of obesity and type 2 diabetes and a method for its extraction. Date of Filing the Patent – 07/12/2018; Indian Patent Application Number – 201831046382; Publication Date (U/S 11A) – 25/01/2019; Inventors - NC Talukdar, Sanjay K Banerjee, Ajay Kumar et al.

    Method of hyperplexing in mass spectrometry to elucidate temporal dynamics of proteome. (Ref No. 201611029904) Inventors – Ajay Kumar, Shilpa Jamwal, Suruchi Aggarwal, Kanury VS Rao, Amit Kumar Yadav.

Preclinical Evaluations of Select AYUSH Herbal Extracts / Formulations for Mitigating SARS- CoV2 and Associated Pathologies

  • Travel grant awarded by Translational Health Science and Technology Institute (THSTI), Faridabad, India to attend EMBL course on “Quantitative Proteomics: Strategies and Tools to Probe Biology” organized by European Molecular Biology Laboratory (EMBL) at EMBL headquarters, Heidelberg, Germany from 19th – 23rd June, 2017. 

  • Travel grant awarded by Council for Scientific and Industrial Research (CSIR), New Delhi, India to attend European Molecular Biology Organization (EMBO) Practical course on “Gene Expression Analysis in Diagnostic Medicine” held at St. Luke’s Medical Centre, Quezon City, Philippines from 17th-26th September, 2006.

  • Travel Grant awarded by National Centre for Biological Sciences (NCBS), Bangalore, India, to attend International Symposium on human origins and genetics entitled “Genes, Evolution and Complex diseases” held at Bangalore, Indiafrom 17th-19th February, 2005.

Present Lab Members

Anvita Chaudhary

Sumedha Mukhi

Manisha Dagar

Roshan Kumar

Amit Kumar

 

Past Lab Members

Alka Kaushal

Surinder Kumar

Pooja Chaudhary