English हिंदी
  • भारत सरकार के विज्ञान एवम प्रौदयोगिकी मंत्रालय के जैव प्रौदयोगिकी विभाग की स्वायत्त संस्थान
  • AN AUTONOMOUS INSTITUTE OF DEPT. OF BIOTECHNOLOGY, MINISTRY OF SCIENCE AND TECHNOLOGY, GOVT OF INDIA
  • होम
  • संकाय प्रोफ़ाइल

DR.YASHWANT KUMAR

Ph.D.

SENIOR RESEARCH SCIENTIST

  • Postdoc, Weizmann Institute of Science, Israel.
  • PhD, National Chemical Laboratory, India.
  • M.Sc., University of Pune.

Our lab focuses on unraveling the pathophysiology of non-alcoholic fatty liver disease (NAFLD) and type 2 diabetes using both human and animal models. We employ liquid chromatography–mass spectrometry (LC–MS) techniques for comprehensive characterization and quantification of metabolites (metabolome), lipids (lipidome), and various exogenous compounds (exposome) during the progression of these diseases. In collaboration with clinicians from institutes across India, we leverage omics approaches to thoroughly investigate the pathology of NAFLD and type 2 diabetes. Moreover, our research emphasizes the development of LC-MS methods, improving data processing, curation of metabolite databases, and the application of advanced statistical methods for omics data analysis. We have established an in-house metabolite database containing over 10,000 compounds, which significantly enhances the accuracy of metabolite identification. Additionally, our work involves elucidating the structure of metabolic pathways and fluxes in diseased conditions using stable isotopes in cell culture and animal models.

Publication
  • Aggarwal H, Gautam J, Kumari D, Gupta SK, Bajpai S, Chaturvedi K, Kumar Y, Dikshit M. Comparative profiling of gut microbiota and metabolome in diet-induced obese and insulin-resistant C57BL/6J mice. Biochim Biophys Acta Mol Cell Res. 2024 Feb;1871(2):119643. doi: 10.1016/j.bbamcr.2023.119643. Epub 2023 Nov 22. PMID: 37996062.
  • Chugh S, Tiwari P, Suri C, Gupta SK, Singh P, Bouzeyen R, Kidwai S, Srivastava M, Rameshwaram NR, Kumar Y, Asthana S, Singh R. Polyphosphate kinase-1 regulates bacterial and host metabolic pathways involved in pathogenesis of Mycobacterium tuberculosis. Proc Natl Acad Sci U S A. 2024 Jan 9;121(2):e2309664121. doi: 10.1073/pnas.2309664121. Epub 2024 Jan 3. PMID: 38170746; PMCID: PMC10786269.
  • Alam MJ, Kamboj P, Sarkar S, Gupta SK, Kasarla SS, Bajpai S, Kumari D, Bisht N, Barge SR, Kashyap B, Deka B, Bharadwaj S, Rahman S, Dutta PP, Borah JC, Talukdar NC, Kumar Y, Banerjee SK. Untargeted metabolomics and phenotype data indicate the therapeutic and prophylactic potential of Lysimachia candida Lindl. towards high-fat high-fructose-induced metabolic syndrome in rats. Mol Omics. 2023 Dec 4;19(10):787-799. doi: 10.1039/d3mo00104k. PMID: 37534494.
  • Kumari D, Gautam J, Sharma V, Gupta SK, Sarkar S, Jana P, Singhal V, Babele P, Kamboj P, Bajpai S, Tandon R, Kumar Y, Dikshit M. Effect of herbal extracts and Saroglitazar on high-fat diet-induced obesity, insulin resistance, dyslipidemia, and hepatic lipidome in C57BL/6J mice. Heliyon. 2023 Nov 4;9(11):e22051. doi: 10.1016/j.heliyon.2023.e22051. PMID: 38027691; PMCID: PMC10663915.
  • Gautam J, Kumari D, Aggarwal H, Gupta SK, Kasarla SS, Sarkar S, Priya MRK, Kamboj P, Kumar Y, Dikshit M. Characterization of lipid signatures in the plasma and insulin-sensitive tissues of the C57BL/6J mice fed on obesogenic diets. Biochim Biophys Acta Mol Cell Biol Lipids. 2023 Sep;1868(9):159348. doi: 10.1016/j.bbalip.2023.159348. Epub 2023 Jun 5. PMID: 37285928.
  • Baygi SF, Kumar Y, Barupal DK. IDSL.CSA: Composite Spectra Analysis for Chemical Annotation of Untargeted Metabolomics Datasets. Anal Chem. 2023 Jun 27;95(25):9480-9487. doi: 10.1021/acs.analchem.3c00376. Epub 2023 Jun 13. PMID: 37311059; PMCID: PMC11080491.
  • Adela R, Kasarla SS, Saquib N, Gupta SK, Bajpai S, Kumar Y, Banerjee SK. Untargeted metabolomics reveals altered branch chain amino acids, glucose and fat metabolism contributing to coronary artery disease among Indian diabetic patients. Mol Omics. 2023 May 9;19(4):321-329. doi: 10.1039/d2mo00320a. PMID: 36752683.
  • Saini V, Mehta D, Gupta S, Kumar S, Rani P, Rana K, Rajput K, Jain D, Pal G, Aggarwal B, Pal S, Gupta SK, Kumar Y, Ramu VS, Bajaj A. Targeting Vancomycin-Resistant Enterococci (VRE) Infections and Van Operon-Mediated Drug Resistance Using Dimeric Cholic Acid-Peptide Conjugates. J Med Chem. 2022 Nov 24;65(22):15312-15326. doi: 10.1021/acs.jmedchem.2c01293. Epub 2022 Nov 4. PMID: 36331380.
  • Kasarla SS, Borse SP, Kumar Y, Sharma N, Dikshit M. In vitro effect of Withania somnifera, AYUSH-64, and remdesivir on the activity of CYP-450 enzymes: Implications for possible herb-drug interactions in the management of COVID-19. Front Pharmacol. 2022 Oct 12;13:973768. doi: 10.3389/fphar.2022.973768. PMID: 36313313; PMCID: PMC9597875.
  • Baygi SF, Banerjee SK, Chakraborty P, Kumar Y, Barupal DK. IDSL.UFA Assigns High-Confidence Molecular Formula Annotations for Untargeted LC/HRMS Data Sets in Metabolomics and Exposomics. Anal Chem. 2022 Oct 4;94(39):13315-13322. doi: 10.1021/acs.analchem.2c00563. Epub 2022 Sep 22. PMID: 36137231; PMCID: PMC9682628.
  • Fakouri Baygi S, Kumar Y, Barupal DK. IDSL.IPA Characterizes the Organic Chemical Space in Untargeted LC/HRMS Data Sets. J Proteome Res. 2022 Jun 3;21(6):1485-1494. doi: 10.1021/acs.jproteome.2c00120. Epub 2022 May 17. PMID: 35579321; PMCID: PMC9177784.
  • Kasarla SS, Garikapati V, Kumar Y, Dodoala S. Interplay of Vitamin D and CYP3A4 Polymorphisms in Endocrine Disorders and Cancer. Endocrinol Metab (Seoul). 2022 Jun;37(3):392-407. doi: 10.3803/EnM.2021.1349. Epub 2022 Jun 3. PMID: 35654576; PMCID: PMC9262690.
  • Das S, Kumar Y, Sharma S, Ray R, Arava S, Seth S, Agarwal A, Sharma G. An Untargeted LC-MS based approach for identification of altered metabolites in blood plasma of rheumatic heart disease patients. Sci Rep. 2022 Mar 28;12(1):5238. doi: 10.1038/s41598-022-09191-z. PMID: 35347173; PMCID: PMC8960827.
  • Rizvi ZA, Dalal R, Sadhu S, Binayke A, Dandotiya J, Kumar Y, Shrivastava T, Gupta SK, Aggarwal S, Tripathy MR, Rathore DK, Yadav AK, Medigeshi GR, Pandey AK, Samal S, Asthana S, Awasthi A. Golden Syrian hamster as a model to study cardiovascular complications associated with SARS-CoV-2 infection. Elife. 2022 Jan 11;11:e73522. doi: 10.7554/eLife.73522. PMID: 35014610; PMCID: PMC8794466.
  • Aggarwal H, Pathak P, Kumar Y, Jagavelu K, Dikshit M. Modulation of Insulin Resistance, Dyslipidemia and Serum Metabolome in iNOS Knockout Mice following Treatment with Nitrite, Metformin, Pioglitazone, and a Combination of Ampicillin and Neomycin. Int J Mol Sci. 2021 Dec 24;23(1):195. doi: 10.3390/ijms23010195. PMID: 35008623; PMCID: PMC8745663.
  • Sarkar S, Kumari D, Gupta SK, Sharma V, Mukhi S, Kamboj P, Sasibhushan V, Rai RK, Jatavallabhula SL, Mahajan D, Kumar Y, Kumar A, Dikshit M. Saroglitazar and Hepano treatment offers protection against high fat high fructose diet induced obesity, insulin resistance and steatosis by modulating various class of hepatic and circulating lipids. Biomed Pharmacother. 2021 Dec;144:112357. doi: 10.1016/j.biopha.2021.112357. Epub 2021 Oct 28. PMID: 34794234.
  • Rizvi ZA, Dalal R, Sadhu S, Kumar Y, Kumar S, Gupta SK, Tripathy MR, Rathore DK, Awasthi A. High-salt diet mediates interplay between NK cells and gut microbiota to induce potent tumor immunity. Sci Adv. 2021 Sep 10;7(37):eabg5016. doi: 10.1126/sciadv.abg5016. Epub 2021 Sep 10. PMID: 34516769; PMCID: PMC8442882.
  • Kamboj P, Sarkar S, Gupta SK, Bisht N, Kumari D, Alam MJ, Barge S, Kashyap B, Deka B, Bharadwaj S, Rahman S, Dutta PP, Borah JC, Talukdar NC, Banerjee SK, Kumar Y. Methanolic Extract of Lysimachia Candida Lindl. Prevents High-Fat High-Fructose-Induced Fatty Liver in Rats: Understanding the Molecular Mechanism Through Untargeted Metabolomics Study. Front Pharmacol. 2021 Apr 15;12:653872. doi: 10.3389/fphar.2021.653872. PMID: 33935766; PMCID: PMC8082144.
  • Pant A, Bag S, Saha B, Verma J, Kumar P, Banerjee S, Kumar B, Kumar Y, Desigamani A, Maiti S, Maiti TK, Banerjee SK, Bhadra RK, Koley H, Dutta S, Nair GB, Ramamurthy T, Das B. Molecular insights into the genome dynamics and interactions between core and acquired genomes of Vibrio cholerae. Proc Natl Acad Sci U S A. 2020 Sep 22;117(38):23762-23773. doi: 10.1073/pnas.2006283117. Epub 2020 Sep 1. PMID: 32873641; PMCID: PMC7519391.
  • Kumar A, Kumar Y, Sevak JK, Kumar S, Kumar N, Gopinath SD. Metabolomic analysis of primary human skeletal muscle cells during myogenic progression. Sci Rep. 2020 Jul 16;10(1):11824. doi: 10.1038/s41598-020-68796-4. PMID: 32678274; PMCID: PMC7366914.
  • Kumari S, Katare PB, Elancheran R, Nizami HL, Paramesha B, Arava S, Sarma PP, Kumar R, Mahajan D, Kumar Y, Devi R, Banerjee SK. Musa balbisiana Fruit Rich in Polyphenols Attenuates Isoproterenol-Induced Cardiac Hypertrophy in Rats via Inhibition of Inflammation and Oxidative Stress. Oxid Med Cell Longev. 2020 Jan 27;2020:7147498. doi: 10.1155/2020/7147498. PMID: 32082481; PMCID: PMC7007945.
  • Nizami HL, Katare P, Prabhakar P, Kumar Y, Arava SK, Chakraborty P, Maulik SK, Banerjee SK. Vitamin D Deficiency in Rats Causes Cardiac Dysfunction by Inducing Myocardial Insulin Resistance. Mol Nutr Food Res. 2019 Sep;63(17):e1900109. doi: 10.1002/mnfr.201900109. Epub 2019 Jun 5. PMID: 31095894.
  • Choudhary E, Sharma R, Kumar Y, Agarwal N. Conditional Silencing by CRISPRi Reveals the Role of DNA Gyrase in Formation of Drug-Tolerant Persister Population in Mycobacterium tuberculosis. Front Cell Infect Microbiol. 2019 Mar 26;9:70. doi: 10.3389/fcimb.2019.00070. PMID: 30972304; PMCID: PMC6443821.
  1. Method of predict diabetes outcome susceptibility (201711041969).
  2. Method of preparing nutraceutical and pharmaceutical products based on Musa balbisiana and uses thereof (201911015320)
  3. A Herbal extract for treatment of obesity and related hyperglycemia and a method for its extraction (201831046382A)
  1. To identify unique differential signatures for the diagnosis of stage-specific Non-alcoholic fatty liver disease using the multi-omics approach: A pilot study. (Co-Principal Investigator)
  2. Metabolic profiling as per glycemic status [normoglycemia/prediabetes/diabetes] in women with a history of Gestational diabetes mellitus (GDM)-A cross-sectional study. (Co-Principal Investigator)
  3. Preclinical Evaluations of Select AYUSH Herbal Extracts / Formulations for Mitigating SARS- CoV2 and Associated Pathologies (Co-Principal Investigator)
  4. Multi-Omics Signatures of Human Placenta: Real time assessment of underlying mechanisms for prediction of birth outcomes and development (Co-Principal Investigator)
  5. Metabolite profiling in pregnant mothers: identification of signature metabolites in pregnancies leading to preterm birth. (Co-Principal Investigator)

 

1. Sonu 

2. Sneh 

3. Sunali 

4. Kartikey

 

 

  • Y[DOT]KUMAR[AT]THSTI[DOT]RES[DOT]IN