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People : Scientific : Faculty & Scientists

DR. TARUN KUMAR SHARMA, Ph.D  

Senior Research Scientist
+91-129-2876370 
tarun [at] thsti [dot] res [dot] in
Ph.D


Areas of Expertise

·       In vitro SELEX and Aptamer Technology

·       Affordable diagnostics

·       Electrochemical sensors and

·       Nanomaterials based Biosensors

 

 

Research Interests/ Research Summary

Our group is primarily focusing on

·      Development of Aptamer based rapid diagnostic test for infectious disease, pollutants and toxins.

·      Application of nanomaterials for diagnostics and therapeutics. 

·      Aptamer based therapeutics 

 For complete list of publication please visit Google Scholar Profile https://scholar.google.co.in/citations?user=7JdrxHsAAAAJ&hl=en

* Represent publication as corresponding author

 Journal publications (*Indicate publication as corresponding author)

 

2018

1. Kalra, P, Mishra S K.; Kaur S.; Prasad, H, P.; Kumar, A.; Sharma, T. K *.; Tyagi J. S*. G-quadruplex forming DNA aptamers inhibit DNA-binding function of HupB (Rv2986c) and Mycobacterium tuberculosis entry into host cells. Molecular Therapy Nucleic Acids (Cell Press) Accepted * as corresponding author

(https://www.sciencedirect.com/science/article/pii/S2162253118302233).

 

2. Dhiman, A.; Haldar S.; Mishra, S.K.; Sharma, N.; Bansal, A.; Ahmad., Y.; Kumar. A.; Sharma, T. K *, Tyagi J. S*. Generation and application of DNA aptamers against HspX for accurate diagnosis of tuberculous meningitis. Tuberculosis (2018), 112, 27–36. * as corresponding author

 

3. Dhiman, A.; Kalra, P, Dhiman A.; Bruno J.G.; Sharma, T. K *. Simple Methods and Rational Design for Enhancing Aptamer Sensitivity and Specificity. Frontiers in Molecular Biosciences (2018) 5:41.doi: 10.3389/fmolb.2018.00041. * as corresponding author

 

4. Kaur H.; Bruno J.G.; Kumar, A.; Sharma, T. K *. Aptamers in the Therapeutics and Diagnostics Pipelines. Theranostics (2018), 8, 4016-4032.* as corresponding author

 

5. Abraham A, Sharma T. K.; Shukla R.; Bansal V. Phytochemicals as dynamic surface ligands to control nanoparticle–protein interactions. ACS Omega (2018), 3, 2220-2229

 

 

2017

1. Sharma, T. K.*; Bruno J.G.; Dhiman, A. ABC of DNA aptamers and related assay developments . Biotechnology Advances (2017), 35 275–301 * as corresponding author

 

2. Dhiman A.; Kalra P.; Bruno J.G.; Sharma, T. K*. Aptamer based point-of-care diagnostic platforms.  Sensors and Actuators B:Chemical (2017), 246 535–553 * as corresponding author

2016

1. Sharma, T. K.*; Bruno J.G.; Cho W. The point behind translation of aptamers for point-of-care diagnostics Aptamers and Synthetic Antibodies, (2016), 2, 36-42. * as corresponding author

2. Gupta M.; Mathur, S.; Sharma, T. K.; Rana M.; Navani N.K.; Pathania, R. A study on metabolic prowess of Pseudomonas sp. RPT 52 to degrade imidacloprid, endosulfan and coragen. J. Hazardous Material (2016), 30, 250-258

2015

1. Sahkir, M.; Jolly, R.; Khan, M. S.; Iram, N.; Sharma, T. K.; Alresayes, S. I. Synthesis and characterization of a nano-hydroxyapatite/chitosan/polyethylene glycol nanocomposite for bone tissue engineering. Polymer Advanced Technologies, 2015, 26, 41-48 DOI: 10.1002/pat.3417

2. Lambadi, P. R.; Sharma, T. K.$; Kumar P.; Vasnani, P.; Mouli, S. R.; Bisht N.; Pathania, R.; Navani, N. K. Facile bio-functionalization of silver nanoparticles for enhanced antibacterial properties, endotoxin removal and biofilm control. International Journal of Nanomedicine 2015,10, 2155-2177. $Joint First Author

3. Sharma, T. K.; Ramanathan. R.; Rakwal, R.; Agrawal, G. K.; Bansal, V. Moving forward in plant food safety and security through NanoBioSensors: Adopt or adapt biomedical technologies? Proteomics 2015, 10, 1680-1692

2014

1. *Sharma, T. K.; Ramanathan. R.;.; Mohammadtaheri, M.; Daima, H. K.; Shukla, R.; Bansal, V. Aptamer-mediated ‘turn-off/turn-on’ nanozyme activity of gold nanoparticles for kanamycin detection. Chemical Communications (Chem Comm), 2014, 50, 15856-15859 DOI-10.1039/C4CC07275H featured on Cover Page * as corresponding author

2. Weerathunge, P Ramanathan. R.; Shukla, R.; *Sharma, T. K.; Bansal, V. Aptamer-controlled reversible inhibition of gold nanozyme activity for pesticide sensing. Analytical Chemistry 2014, 86 (24), pp 11937–11941. DOI: 10.1021/ac5028726 featured on Cover Page * as corresponding author

3. Chopra, A.; Shukla, R.; *Sharma, T. K. Aptamers as an emerging player in Biology. Aptamers and Synthetic Antibodies 2014, 1(1): 1-11). Inaugural issue. * as corresponding author

4. *Sharma, T. K.; Shukla, R. Nucleic acid aptamers as an emerging diagnostic tool for animal pathogens. Advances in Animal and Veterinary Sciences 2014, 2(1) 50-55. * as corresponding author

5. *Sharma, T. K.; Shukla, R. Editorial Nanoscience and Aptamer Technology for Point of Care Diagnostics. Nanoscience and Technology 2014, 1(2) 1-2. Editorial

2013

1. Ghosh, I. N.; Patil , S.D. Sharma, T. K.; Shrivastava, S. K.; Pathania, R.; Navani, N. K. Synergistic action of cinnamaldehyde with silver nanoparticles against spore-forming bacteria: a case for judicious use of silver nanoparticles for antibacterial applications. International Journal of Nanomedicine 2013, 8: 4721

2012

1. Lange, M. J.; Sharma, T. K.; Whatley, A. S.; Landon, L. A.; Tempesta, M. A.; Johnson M. C.; Burke, D. H. Robust suppression of HIV replication by intracellularly expressed reverse transcriptase aptamers is independent of ribozyme processing. Molecular Therapy, 2012, 20(12): 2304-2314. IF-6.82

2. Sharma, T. K.; Sapra M.; Chopra A.; Sharma R.; Malik, M.; Pathania, R.; Navani, N. K.; Interaction of bacteriocin-capped silver nanoparticles with food pathogens and their antibacterial effect. International Journal of Green Nanotechnology, 2012, 4(2), 93-110.

3. Sharma, T. K.; Chopra A.; Sapra M.; Kumanwat D.; Pathania, R.; Navani, N. K.; Green synthesis and antimicrobial potential of silver nanoparticles. International Journal of Green Nanotechnolog

1. Weerathunge, P.; Sharma, T. K.; Ramanathan, R.; Bansal, V.;Nanozyme-based environmental Monitoring. Advanced Environmental Analysis: Applications of Nanomaterials, Volume 2 Chapter: 23 (Royal Society of Chemistry  UK)-2016

2.   Chopra, A.; Sharma, T. K.; Pathania,  R.; Navani, N. K. Development of an aptamer- nanoparticles based nanobiosensor for bacterial detection. Sinha, S., Navani, N. K. (Eds.), Studium Press-USA, 2013, Volume 10, Chapter 4,  (ISBN: 1-62699-000-10).

3.    Sapra, M.; Sharma, T. K.; Pathania,  R.; Navani, N. K.  Nanoparticles based optical detection of xenobiotics and small molecules. Sinha, S., Navani, N. K. (Eds.), Studium Press-USA, 2013, Volume 10, Chapter 8,  (ISBN: 1-62699-000-10).

4.    *Sharma, T. K.; Sharma, K. K.  Pesticide in soil environment and their microbial degradation. Bharti, P. K., Zaki, M. S. (Eds.), Discovery publishing house-India, 2013, Chapter 6, (ISBN: 9-5056-313-4).

 

1.Tarun K Sharma, Ritu Das, A.Dhiman, Jaya S. Tyagi. Aptamer against M.tb MPT51 and uses thereof. Patent Number 201811023360

2. Tarun K Sharma, Priya Kalra, H.K. Prasad Jaya S. Tyagi. Novel DNA aptamers against nucleoid-associated protein HupB of Mycobacterium tuberculosis and uses thereof. Patent Number 201711001246

3. Tarun K Sharma, Jaya S. Tyagi, Abhijeet Dhiman, Chanchal Kumar, Ishara Datta. aptamer against mycobacterial malate synthase and uses thereof. Patent Number 201611021901

4. Tarun K Sharma, Jaya S. Tyagi, Abhijeet Dhiman. Novel DNA aptamers against heat shock protein HspX of mycobacterium tuberculosis and uses thereof. Patent Number 201611001550 

1. Title of grant: Affordable, portable, easy to use rapid and accurate diagnostic test for pulmonary tuberculosis

Funding Agency: Bill and Melinda Gates Foundation under Grand Challenges Exploration scheme

Amount: Rs. 50 lakhs

2. Title of grant: Aptamer based rapid test to differentiate venomous snake bites from non-venomous and dry snake bites.- as PI (Industry-academia collaboration)

Funding Agency: BIRAC under PACE scheme

Amount: Rs. 48.65 lakhs (total grant); Sanctioned to THSTI 36.40 Lakhs.

3. Title of grant:  Aptamer-based Tuberculosis Diagnostics Toolbox - as PI (Industry-academia collaboration)

Funding Agency: DBT under call for TB diagnostics

Amount: Rs. 65 lakhs (total grant) Sanctioned to THSTI 35 Lakhs.

4. Title of grant: Aptamer based rapid diagnosis of snake bite: an unmet Indian need- as PI

Funding Agency: DBT under Innovative Young Biotechnologist Award

Amount: Rs. 66 lakhs

5. Title of grant: Mycobacterium tuberculosis antigen-detection based point-of-care test using aptamer technology for tuberculous meningitis (TB meningitis)- as PI (Industry-academia collaboration)

Funding Agency: BIRAC

Amount: Rs. 50 lakhs

 

2016          DBT Innovative Young Biotechnologist Award (IYBA)

2014          Australian Endeavour Research Award (Commonwealth of Australia)

2014          Indo-Australian Career Boosting Research Fellowship (DBT-India and Australian Research Council)-Not Availed

2014          Indo-Finnish Diagnostic Fellowship (DBT-India and Government of Finland) -Not Availed

2012          Innovation Award, Department of Biotechnology- Govt. of India

2010          Visiting Research Fellowship- National Institute of Health- USA

2009          National Doctoral Fellowship (AICTE-India)

2007          National Eligibility Test (Eligibility for Lectureship) cum Junior Research Fellowship

 


MS. ANJALI ANAND
TECHNICAL ASSISTANT

DR. BANDHAN CHATTERJEE
POST DOCTORAL FELLOW

MR. SOON JYOTI DAS
JUNIOR RESEARCH FELLOW

DR. NEETI KALYANI
POST DOCTORAL FELLOW
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