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People : Scientific : Faculty & Scientists

DR. TARUN KUMAR SHARMA, Ph.D 

Iyba Fellow
+91-129-2876370 
tarun [at] thsti [dot] res [dot] in
Ph.D

Areas of Expertise

·       In vitro SELEX and Aptamer Technology

·       Affordable diagnostics

·       Electrochemical sensors and

·       Nanomaterials based Biosensors

 

 

Research Interests/ Research Summary

Our group is primarily focusing on

·      Development of Aptamer based rapid diagnostic test for infectious disease, pollutants and toxins.

·      Application of nanomaterials for diagnostics and therapeutics. 

·      Aptamer based therapeutics 

 For complete list of publication please visit Google Scholar Profile https://scholar.google.co.in/citations?user=7JdrxHsAAAAJ&hl=en

* Represent publication as corresponding author

 Journal publications (*Indicate publication as corresponding author)

 

2018

1. Kalra, P, Mishra S K.; Kaur S.; Prasad, H, P.; Kumar, A.; Sharma, T. K *.; Tyagi J. S*. G-quadruplex forming DNA aptamers inhibit DNA-binding function of HupB (Rv2986c) and Mycobacterium tuberculosis entry into host cells. Molecular Therapy Nucleic Acids (Cell Press) Accepted * as corresponding author

(https://www.sciencedirect.com/science/article/pii/S2162253118302233).

 

2. Dhiman, A.; Haldar S.; Mishra, S.K.; Sharma, N.; Bansal, A.; Ahmad., Y.; Kumar. A.; Sharma, T. K *, Tyagi J. S*. Generation and application of DNA aptamers against HspX for accurate diagnosis of tuberculous meningitis. Tuberculosis (2018), 112, 27–36. * as corresponding author

 

3. Dhiman, A.; Kalra, P, Dhiman A.; Bruno J.G.; Sharma, T. K *. Simple Methods and Rational Design for Enhancing Aptamer Sensitivity and Specificity. Frontiers in Molecular Biosciences (2018) 5:41.doi: 10.3389/fmolb.2018.00041. * as corresponding author

 

4. Kaur H.; Bruno J.G.; Kumar, A.; Sharma, T. K *. Aptamers in the Therapeutics and Diagnostics Pipelines. Theranostics (2018), 8, 4016-4032.* as corresponding author

 

5. Abraham A, Sharma T. K.; Shukla R.; Bansal V. Phytochemicals as dynamic surface ligands to control nanoparticle–protein interactions. ACS Omega (2018), 3, 2220-2229

 

 

2017

1. Sharma, T. K.*; Bruno J.G.; Dhiman, A. ABC of DNA aptamers and related assay developments . Biotechnology Advances (2017), 35 275–301 * as corresponding author

 

2. Dhiman A.; Kalra P.; Bruno J.G.; Sharma, T. K*. Aptamer based point-of-care diagnostic platforms.  Sensors and Actuators B:Chemical (2017), 246 535–553 * as corresponding author

2016

1. Sharma, T. K.*; Bruno J.G.; Cho W. The point behind translation of aptamers for point-of-care diagnostics Aptamers and Synthetic Antibodies, (2016), 2, 36-42. * as corresponding author

2. Gupta M.; Mathur, S.; Sharma, T. K.; Rana M.; Navani N.K.; Pathania, R. A study on metabolic prowess of Pseudomonas sp. RPT 52 to degrade imidacloprid, endosulfan and coragen. J. Hazardous Material (2016), 30, 250-258

2015

1. Sahkir, M.; Jolly, R.; Khan, M. S.; Iram, N.; Sharma, T. K.; Alresayes, S. I. Synthesis and characterization of a nano-hydroxyapatite/chitosan/polyethylene glycol nanocomposite for bone tissue engineering. Polymer Advanced Technologies, 2015, 26, 41-48 DOI: 10.1002/pat.3417

2. Lambadi, P. R.; Sharma, T. K.$; Kumar P.; Vasnani, P.; Mouli, S. R.; Bisht N.; Pathania, R.; Navani, N. K. Facile bio-functionalization of silver nanoparticles for enhanced antibacterial properties, endotoxin removal and biofilm control. International Journal of Nanomedicine 2015,10, 2155-2177. $Joint First Author

3. Sharma, T. K.; Ramanathan. R.; Rakwal, R.; Agrawal, G. K.; Bansal, V. Moving forward in plant food safety and security through NanoBioSensors: Adopt or adapt biomedical technologies? Proteomics 2015, 10, 1680-1692

2014

1. *Sharma, T. K.; Ramanathan. R.;.; Mohammadtaheri, M.; Daima, H. K.; Shukla, R.; Bansal, V. Aptamer-mediated ‘turn-off/turn-on’ nanozyme activity of gold nanoparticles for kanamycin detection. Chemical Communications (Chem Comm), 2014, 50, 15856-15859 DOI-10.1039/C4CC07275H featured on Cover Page * as corresponding author

2. Weerathunge, P Ramanathan. R.; Shukla, R.; *Sharma, T. K.; Bansal, V. Aptamer-controlled reversible inhibition of gold nanozyme activity for pesticide sensing. Analytical Chemistry 2014, 86 (24), pp 11937–11941. DOI: 10.1021/ac5028726 featured on Cover Page * as corresponding author

3. Chopra, A.; Shukla, R.; *Sharma, T. K. Aptamers as an emerging player in Biology. Aptamers and Synthetic Antibodies 2014, 1(1): 1-11). Inaugural issue. * as corresponding author

4. *Sharma, T. K.; Shukla, R. Nucleic acid aptamers as an emerging diagnostic tool for animal pathogens. Advances in Animal and Veterinary Sciences 2014, 2(1) 50-55. * as corresponding author

5. *Sharma, T. K.; Shukla, R. Editorial Nanoscience and Aptamer Technology for Point of Care Diagnostics. Nanoscience and Technology 2014, 1(2) 1-2. Editorial

2013

1. Ghosh, I. N.; Patil , S.D. Sharma, T. K.; Shrivastava, S. K.; Pathania, R.; Navani, N. K. Synergistic action of cinnamaldehyde with silver nanoparticles against spore-forming bacteria: a case for judicious use of silver nanoparticles for antibacterial applications. International Journal of Nanomedicine 2013, 8: 4721

2012

1. Lange, M. J.; Sharma, T. K.; Whatley, A. S.; Landon, L. A.; Tempesta, M. A.; Johnson M. C.; Burke, D. H. Robust suppression of HIV replication by intracellularly expressed reverse transcriptase aptamers is independent of ribozyme processing. Molecular Therapy, 2012, 20(12): 2304-2314. IF-6.82

2. Sharma, T. K.; Sapra M.; Chopra A.; Sharma R.; Malik, M.; Pathania, R.; Navani, N. K.; Interaction of bacteriocin-capped silver nanoparticles with food pathogens and their antibacterial effect. International Journal of Green Nanotechnology, 2012, 4(2), 93-110.

3. Sharma, T. K.; Chopra A.; Sapra M.; Kumanwat D.; Pathania, R.; Navani, N. K.; Green synthesis and antimicrobial potential of silver nanoparticles. International Journal of Green Nanotechnolog

1. Weerathunge, P.; Sharma, T. K.; Ramanathan, R.; Bansal, V.;Nanozyme-based environmental Monitoring. Advanced Environmental Analysis: Applications of Nanomaterials, Volume 2 Chapter: 23 (Royal Society of Chemistry  UK)-2016

2.   Chopra, A.; Sharma, T. K.; Pathania,  R.; Navani, N. K. Development of an aptamer- nanoparticles based nanobiosensor for bacterial detection. Sinha, S., Navani, N. K. (Eds.), Studium Press-USA, 2013, Volume 10, Chapter 4,  (ISBN: 1-62699-000-10).

3.    Sapra, M.; Sharma, T. K.; Pathania,  R.; Navani, N. K.  Nanoparticles based optical detection of xenobiotics and small molecules. Sinha, S., Navani, N. K. (Eds.), Studium Press-USA, 2013, Volume 10, Chapter 8,  (ISBN: 1-62699-000-10).

4.    *Sharma, T. K.; Sharma, K. K.  Pesticide in soil environment and their microbial degradation. Bharti, P. K., Zaki, M. S. (Eds.), Discovery publishing house-India, 2013, Chapter 6, (ISBN: 9-5056-313-4).

 

1.Tarun K Sharma, Ritu Das, A.Dhiman, Jaya S. Tyagi. Aptamer against M.tb MPT51 and uses thereof. Patent Number 201811023360

2. Tarun K Sharma, Priya Kalra, H.K. Prasad Jaya S. Tyagi. Novel DNA aptamers against nucleoid-associated protein HupB of Mycobacterium tuberculosis and uses thereof. Patent Number 201711001246

3. Tarun K Sharma, Jaya S. Tyagi, Abhijeet Dhiman, Chanchal Kumar, Ishara Datta. aptamer against mycobacterial malate synthase and uses thereof. Patent Number 201611021901

4. Tarun K Sharma, Jaya S. Tyagi, Abhijeet Dhiman. Novel DNA aptamers against heat shock protein HspX of mycobacterium tuberculosis and uses thereof. Patent Number 201611001550 

1. Title of grant: Affordable, portable, easy to use rapid and accurate diagnostic test for pulmonary tuberculosis

Funding Agency: Bill and Melinda Gates Foundation under Grand Challenges Exploration scheme

Amount: Rs. 50 lakhs

2. Title of grant: Aptamer based rapid test to differentiate venomous snake bites from non-venomous and dry snake bites.- as PI (Industry-academia collaboration)

Funding Agency: BIRAC under PACE scheme

Amount: Rs. 48.65 lakhs (total grant); Sanctioned to THSTI 36.40 Lakhs.

3. Title of grant:  Aptamer-based Tuberculosis Diagnostics Toolbox - as PI (Industry-academia collaboration)

Funding Agency: DBT under call for TB diagnostics

Amount: Rs. 65 lakhs (total grant) Sanctioned to THSTI 35 Lakhs.

4. Title of grant: Aptamer based rapid diagnosis of snake bite: an unmet Indian need- as PI

Funding Agency: DBT under Innovative Young Biotechnologist Award

Amount: Rs. 66 lakhs

5. Title of grant: Mycobacterium tuberculosis antigen-detection based point-of-care test using aptamer technology for tuberculous meningitis (TB meningitis)- as PI (Industry-academia collaboration)

Funding Agency: BIRAC

Amount: Rs. 50 lakhs

 

2016          DBT Innovative Young Biotechnologist Award (IYBA)

2014          Australian Endeavour Research Award (Commonwealth of Australia)

2014          Indo-Australian Career Boosting Research Fellowship (DBT-India and Australian Research Council)-Not Availed

2014          Indo-Finnish Diagnostic Fellowship (DBT-India and Government of Finland) -Not Availed

2012          Innovation Award, Department of Biotechnology- Govt. of India

2010          Visiting Research Fellowship- National Institute of Health- USA

2009          National Doctoral Fellowship (AICTE-India)

2007          National Eligibility Test (Eligibility for Lectureship) cum Junior Research Fellowship