People : Scientific : Faculty & Scientists

DR. MILAN SURJIT, Ph.D. 

Associate Professor
+91-129-3087318  (+91-129-2876317)
milan [at] thsti [dot] res [dot] in
Post-Doctoral Research, Programme in Functional Genomics and Cancer, Institute of Genetics and Molecular and Cellular Biology, Strasbourg, France, (Advisor: Prof. Pierre Chambon).
PhD. Molecular Biology (Virology), International Centre for Genetic Engineering and Biotechnology, New Delhi, India.
MSc. Zoology, Banaras Hindu University, Varanasi, India.

Present Research Interest

Understanding the biology of Hepatitis E virus and development of vaccine and drugs against it.

Research Summary

Hepatitis E Virus (HEV) causes acute hepatitis in human characterized by jaundice, anorexia, nausea, abdominal pain, malaise, fever and hepatomegaly. In India, it accounts for ~50% of sporadic hepatitis. A recent report also suggests chronic HEV infection in ~60% liver transplantation patients. No vaccine or drug is yet available against HEV in India and most of the world.

My laboratory is investigating  multiple aspects of HEV biology to generate knowledge and resources essential for minimizing or eradicating HEV induced clinical complications in human. My current research plan involves (a) development of a mouse model that mimics HEV infection in human and use this model to characterize the molecular mechanism of HEV pathogenesis (b) understanding the host-pathogen interactions involved in HEV pathogenesis (c) understanding the mechanism of viral translation and replication (d) identification of novel anti-HEV compounds that can be used as therapeutic drugs and (e) development of a recombinant vaccine against HEV.

 

Research Articles
  • Kaushik N, Subramani C, Anang S, Muthumohan R, Shalimar, Nayak B, Ranjith-Kumar CT, Surjit M. 2017. Zinc Salts Block Hepatitis E Virus Replication by Inhibiting the Activity of Viral RNA-Dependent RNA Polymerase. J Virol. 91, 00754-17.

  • Madhvi A, Hingane S, Srivastav R, Joshi N, Subramani C, Muthumohan R, Khasa R, Varshney S, Kalia M, Vrati S, Surjit M, Ranjith-Kumar CT. 2017. A screen for novel hepatitis C virus RdRp inhibitor identifies a broad-spectrum antiviral compound. Sci Rep. 7, 5816.

  • Ganti KP, Mukherji A, Surjit M, Li M, Chambon P. 2017. Similarities and differences in the transcriptional control of expression of the mouse TSLP gene in skin epidermis and intestinal epithelium. Proc Natl Acad Sci U S A. 114, E951-E960.

  • Wang W, Hakim MS, Nair VP, de Ruiter PE, Huang F, Sprengers D, Van Der Laan LJ, Peppelenbosch MP, Surjit M, Pan Q. 2016. Distinct Antiviral Potency of Sofosbuvir Against Hepatitis C and E Viruses. Gastroenterology. 151, 1251-1253.

  • Nair VP, Anang S*, Subramani C*, Madhvi A, Bakshi K, Srivastava A, Shalimar, Nayak B, Ranjith Kumar CT, Surjit M. 2016. Endoplasmic Reticulum Stress Induced Synthesis of a Novel Viral Factor Mediates Efficient Replication of Genotype-1 Hepatitis E Virus. PLoS Pathog 12(4): e1005521.

  • Anang S, Subramani C*, Nair VP*, Kaul S, Kaushik N, Sharma C, Tiwari A, Ranjith Kumar CT, Surjit M. 2016. Identification of critical residues in Hepatitis E virus macro domain involved in its interaction with viral methyltransferase and ORF3 proteins. Sci. Rep. 6, 25133.
  • Surjit M, Ganti KP*, Mukherji A*, Tao Y, Hua G, Metzger D, Li M, Chambon P. 2011. Widespread negative response elements mediate direct repression by agonist-liganded glucocorticoid receptor. Cell. 145, 224-241.
  • Surjit M, Kumar R, Mishra RN, Reddy MK, Chow VT, Lal SK. 2005. The SARS coronavirus nucleocapsid (N) protein is phosphorylated and localizes in the cytoplasm by 14-3-3 mediated translocation. J Virol. 79,11476-11486.
  • Surjit M, Jameel S, Lal SK. 2004. The ORF2 protein of hepatitis E virus binds the 5' region of viral RNA. J Virol. 78, 320-328.
  • Surjit M, Liu B, Kumar P, Chow VT, Lal SK. 2004. The nucleocapsid protein of the SARS coronavirus is capable of self-association through a C-terminal 209 amino acid interaction domain. Biochem Biophys Res Commun. 317, 1030-1036.
  • Tyagi S, Surjit M, Lal SK. 2005. The ORF3 protein of Hepatitis E Virus interacts with Bikunin, a Kunitz-type serine protease inhibitor, using its 41 amino acid C-terminal region. J Virol. 79, 12081-12087.
  • Tyagi S, Surjit M, Roy AK, Jameel S, Lal SK. 2004. The ORF3 protein of hepatitis E virus interacts with liver-specific alpha1-microglobulin and its precursor alpha1-microglobulin/bikunin precursor (AMBP) and expedites their export from the hepatocyte. J Biol Chem. 279, 29308-29319.

* Equal contributors

Review Articles
Book Chapters
  • M. Surjit and S. K. Lal. 2010. ORF3 protein of Hepatitis E virus: possible structural and regulatory functions during viral pathogenesis. (eds. D. Raghunath & V. Nagaraja) Mechanisms of Microbial Pathogenesis. Macmillan, Bangalore. p 168-182.
  • M. Surjit and S. K. Lal. 2010. The nucleocapsid protein of the SARS Coronavirus: Structure, Function and Therapeutic potential. (ed. S. K. Lal) Molecular Biology of the SARS-Coronavirus. Springer. Germany. p129-151.
  • M. Surjit, Bhavna Varshney and S. K. Lal. 2006. Molecular biology of the Severe Acute respiratory Syndrome (SARS) Coronavirus. (ed. T. Mizutani) Molecular Biology of the SARS-Coronavirus. Transworld Research Network, India. p1-16.

Published Protocols

  • Nair VP, Surjit M. 2017. RNA Strand Displacement Assay for Hepatitis E Virus Helicase. Bio-protocol. 7, e2198.
  • Nair VP, Anang S, Srivastava A, Surjit M. 2017. RNA-dependent RNA Polymerase Assay for Hepatitis E Virus. Bio-protocol. 7, e2199.
  • Ramalingaswamy re-entry fellowship - 2011, Department of Biotechnology
  • Ramanujan fellowship - 2011, Department of Science and Technology

 

Current members

Mr S. Chandru (graduate student)

Ms Saumya Anang (graduate student)

Ms Nidhi Kaushik  (graduate student)

Mr Shiv kumar  (graduate student)

Dr Madhu pareek (Technical Officer I)

Dr Jyoti Gupta (Post-doctoral fellow)

Mr Jayveer Singh (Lab attendant)

 

Past members                                                     Current position

Mr Sumeet Jain (Msc trainee)                                    Graduate student, Institute of Life Science, Bhubaneswar, India

Ms Karishma Bakshi (Junior research Fellow)           Graduate student, Shiv Nadar University, Dadri, India

Ms Akriti Shrivastava (Junior research Fellow)          Graduate student, Shiv Nadar University, Dadri, India    

Ms Vidya P. Nair (Senior research fellow)                  Graduate student, Technical University of Munich, Munich, Germany

Mr Ravi Jain  (Junior research Fellow)                       Graduate student, Shiv Nadar University, Dadri, India  

Mr Soumajeet Mukherjee (Junior research fellow)     Junior research fellow, THSTI, Faridabad, India

Ms Sheetal Kaul (Junior research fellow)                   Senior research fellow, ICGEB, New Delhi, India

Ms Anshu Sharma (MSc. trainee)