People : Scientific : Faculty & Scientists

DR. GAURAV BATRA, Ph.D. 

Assistant Professor
+91-129-2876357  (1292876357)
gaurav.batra [at] thsti [dot] res [dot] in
Postdoctoral Research, Department of Biotechnology, University of Turku, Finland
Ph.D. International Centre for Genetic Engineering and Biotechnology, New Delhi, India
PG Diploma in Intellectual Property Rights Laws from Indian Law Institute, New Delhi, India
M.Sc. Biotechnology, Bundelkhand University, Jhansi

Present Research Interest

Knowing the cause responsible for a patient’s illness remains fundamental to evidence based treatment and care. Despite the fact that the reliable diagnostic tools affect health care decisions to a degree well out of proportion to their cost, in the developing world healthcare workers are forced to use empirical approaches of treatment which often result in inappropriate clinical outcomes because of lack of reliable, affordable and practical in vitro diagnostic solutions.

Goal of my lab is to provide diagnostic solutions which are reliable in local conditions, affordable and practical. Major focus of our work is on the development of high quality diagnostic assays for acute febrile illnesses (e.g. dengue, malaria, typhoid, scrub typhus etc) and blood borne infections (e.g. HIV, HCV and HBV). To generate high quality diagnostic intermediates, we are involved in protein and antibody engineering, cost effective recombinant antigen and antibody production in E. coli and yeast Pichia pastoris, expression host engineering and high throughput clone screening. We are using genome fragment phage display libraries and antibody libraries for immune epitope mapping and biomarker discovery.

Major scientific contribution

At ICGEB, I was involved in the development of diagnostic intermediates and assays for the detection of anti-dengue virus IgG, IgM antibodies and NS1 antigen. This “know-how” was transferred to a diagnostic company which resulted in a very successful commercial product for detection of dengue NS1 antigen & differential detection of IgM & IgG antibodies in Human Serum/ Plasma.

Publications

  • Kaushik, N., Rohila, D., Arora, U., Raut, R., Lamminmaki, U., Khanna, N., and Batra, G. (2016). Casamino acids facilitate the secretion of recombinant dengue virus serotype-3 envelope domain III in Pichia pastoris. BMC Biotechnol 16, 12.
  • Batra, G.,Nemani, S.K., Tyagi, P., Swaminathan, S., Khanna, N. (2011) Evaluation of envelope domain III-based single chimeric tetravalent antigen and monovalent antigen mixtures for the detection of anti-dengue antibodies in human sera. BMC Infect Dis.11, 64.
  • Batra,G., Gurramkonda,C., Nemani,S.K., Jain,S.K., Swaminathan,S. and Khanna, N.(2010) Optimization of conditions for secretion of dengue virus type 2 envelope domain III using Pichia pastoris, J. Biosci. Bioeng., 110, 408-14.
  • Batra,G.,Talha,S.M., Nemani,S.K., Dhar,N., Swaminathan,S. and Khanna,N. (2010) Expression, purification and characterization of in vivo biotinylated dengue virus envelope domain III based tetravalent antigen. Protein Expr. Purif., 74, 99–105.
  • Batra,G.,Raut,R., Dahiya,S., Kamran,N., Swaminathan,S. and Khanna,N. (2010) Pichia pastoris-expressed dengue virus type 2 envelope domain III elicits virus-neutralizing antibodies. J Virol. Methods., 167, 10-16.
  • Swaminathan,S.,Batra,G. and Khanna,N. (2010) Dengue vaccines: state of the art. Expert. Opin. Ther. Pat., 20, 819-835.
  • Etemad,B.,Batra,G.,Raut,R., Dahiya,S., Khanam,S., Swaminathan,S. and Khanna,N. (2008) An envelope domain III-based chimeric antigen produced in Pichia pastoris elicits neutralizing antibodies against all four dengue virus serotypes. Am J Trop Med Hyg, 79, 353-363.
  • Hapugoda,M.D.,Batra,G., Abeyewickreme,W., Swaminathan,S. and Khanna,N. (2007) Single antigen detects both immunoglobulin M (IgM) and IgG antibodies elicited by all four dengue virus serotypes. Clin. Vaccine Immunol., 14, 1505-1514.
  • Batra,G., Chauhan,V.S., Singh,A., Sarkar,N.K., Grover,A. (2007) Complexity of rice Hsp100 gene family: lessons from rice genome sequence data. J Biosci, 32(3):611-9.

Patent

  • Batra,G., Hapugoda,M.D., Chaudhary,S., Swaminathan,S. and Khanna,N., Tetravalent dengue specific domain III based chimeric recombinant protein, WO/2007/034507
  • Marie Curie International Fellow award by European Commission in 2011.