People : Scientific : Faculty & Scientists

DR. BHABATOSH DAS, M.Tech., Ph.D. 

Assistant Professor
+91-129-2876471 
bhabatosh [at] thsti [dot] res [dot] in
2012 - Present: Assistant Professor
2008 - 2011: Post-Doctoral Research Fellow, Department of Chromosome Segregation and Cell Division, CGM-CNRS, Gif, France
2003 - 2008: Ph.D. (Science), Indian Institute of Chemical Biology, Jadavpur University, India
2001 - 2003: M.Tech. (Biotech. and Biochem. Eng.), IIT-Kharagpur, India

 Research of Interest

The main objective of my laboratory is to explore the striking alliance between microorganisms and the human host and attempt to understand the role and effect of microorganisms in human health and diseases. Currently, we are investigating (i) The role and impact of human gut microbiota in Severe Acute Malnutrition (SAM) and Inflammatory Bowl Diseases (IBD), which are directly linked to microbial richness, dynamics, and functional repository of the microbial genome. We adopted culture independent metagenomics approaches to understand the importance of microbial community as a whole rather than as individual organisms as in the case of microbial pathogens. (ii) Our second major research interest is to explore the functional repertoires of human gut microbiota by creating fosmid libraries of the gut microbial metagenome. We wish to understand the mechanisms that connenct the human gut microbiota including pathobionts to the efficacy and toxicity of xenobiotics, including antimicrobial drugs and complex dietary compounds.

SELECTED PEER-REVIEWED PUBLICATIONS

2017

  • Das B@., Choudhuri S., Srivastava R., Nair G.B., Ramamurthy T. Fostering research on antimicrobial resistance in India. BMJ 2017;358:j3535 PMID:28877874
  • Bag S., Ghosh T.S., Das B@. Complete genome sequence of Faecalibacterium prausnitzii strain Indica isolated from the gut of a healthy adult Indian. Genome Announc. 16;5(46). pii: e01286-17.
  • Bag S., Ghosh T.S., Das B@. Complete genome sequence of Collinsella aerofaciens strain Indica isolated from the gut of a healthy Indian. Genome Announc. (Accepted)
  • Kumar P., Bag S., Dey P., Ghosh T.S., Dayal M., Saha B., Verma J., Saxena S., Pant A., Kumar D., Desigamani A., Rana P., Hanpude P., Maity T.K., Bhadra R.K., Sharma N.C., Nair G.B., Ramamurthy T., Das B. Molecular Insights into Antimicrobial Resistance Traits of Multidrug Resistant Enteric Pathogens isolated from India Sci.  Rep.  2017 Oct 31;7(1):14468. PMID: 29089611
  • Bag S., Ghosh T.S., Das B@. Whole Genome Sequence of Megasphaera elsdenii strain Indica isolated from the gut of a healthy adult Indian. Genome Announc 5: e01033-17.
  • Das A., Pereira V., Saxena S., Ghosh TS., Anbumani D., Bag S., Das B., Nair GB., Abraham P., Mande SS (2017) Gastric microbiome of Indian patients with Helicobacter pylori infection, and their interaction network. 2017, Sci.  Rep. 7, 15438. doi:10.1038/s41598-017-15510-6.
  • Bag S., Ghosh T.S., Das B@. Whole Genome Sequence of Bifidobacterium longum strain Indica isolated from the gut of a healthy adult Indian. Genome Announc. 5: e01017-17.
  • Srivastava N., Shelly A., Kumar M., Pant A., Das B., Majumdar T., Mazumder S. Aeromonas hydrophila utilizes TLR4 topology for synchronous activation of MyD88 and TRIF to orchestrate anti-inflammatory responses in zebrafish. Cell Death Discv. 2017, 3, 17067.
  • Bag S., Ghosh T., Das B.@ Draft Genome Sequence of Prevotella copri isolated from the gut of a healthy adult Indian. Genome Announc. 5:e00834-17. PMID:28912310

 

2016

  • Das B., Pazhani G.P., Sarkar A., Mukhopadhyay A.K., Nair G.B., Ramamurthy T. Molecular Evolution and Functional Divergence of Vibrio cholerae. Curr. Opin. Infect. Dis. 2016, 29(5):520-7. PMID:27537830
  • Bag S.*, Saha B.*, Mehta O.*, D. Anbumani, Kumar N., Dayal M., Pant A., Kumar P., Saxena S., AllinK.H., Hansen T., Arumugam M., Vestergaard H.,Pedersen O., Pereira V., Abraham P., Tripathi R., Wadhwa N., Bhatnagar S., PrakashV.G., Radha V., Anjana R. M., Mohan V., Takeda K., Kurakawa T., Nair G.B., Das B.@ An Improved Method For Isolation Of High Quality Community Bacterial DNA From Human And Environmental Samples For Metagenomics Studies. Sci. Rep. 2016, 6, 26775
  •  Rakonjac J., Das B., and Derda R. Filamentous Bacteriophage in Bio/Nano/Technology, Bacterial Pathogenesis and Ecology. Front. Microbiol. 2016, 7:2109 PMID:28066406.
  • Chowdhury G., Bhadra R.K., Bag S., Pazhani G.P., Das B., Basu P., Nagamani K., Nandy R.K., Mukhopadhyay A.K., Ramamurthy T.  Rugose atypical Vibrio cholerae O1 El Tor responsible for 2009 cholera outbreak in India. J. Med. Microb. 2016, 65: 1130-1136 PMID:27561681

2015

  • Pant A., Anbumani D., Bag S., Mehta B., Kumar P., Saxena S., Nair G. B., Das B.@ Effect of LexA on chromosomal integration of CTXfin Vibrio cholerae. J. Bacteriol.2015, 198:268-75. PMID: 26503849

2014

  • Das B.@ Mechanistic Insights Into Filamentous Phage Integration In Vibrio cholerae. Front. Microbiol. 2014, 5:650. PMID:25506341
  • Das B.@, Kumari R., Pant A., Sen Gupta S., Saxena S., Nair G.B. A Novel, Broad-Range, CTXΦ-Derived Stable Integrative Expression Vector For Functional Studies. J. Bacteriol. 2014, 196(23): 4071-80. PMID:25225263
  • Das B*, Banerjee R*, Nair G.B., Basak S. Dynamics in genome evolution of Vibrio cholerae. Infect Genet Evol. 2014, 23:32-41. PMID:24462909 (*equally contributed)
  • Das B.*,Midonet C.*,  Paly E., Barre F.X. XerD initiates the integration of TLCΦinto the Vibrio cholerae genome independently of FtsK. Proc. Natl. Acad. Sci. USA; 2014, 111 (47): 16848-16853.PMID:25385643  (*equally contributed)
  •  Das B., Nair G.B., Bhadra R.K. Acquisition and dissemination mechanisms of CTXΦ in Vibrio cholerae: New paradigm for dif residents. World J Med Genet. 2014, 4: 27-33.
  • Bag S., Das B., Dasgupta S., Bhadra R.K. Mutational analysis of the (p)ppGpp synthetase activity of the Rel enzyme of Mycobacterium tuberculosis. Arch Microbiol. 2014,196(8):575-88. PMID:24859914
  • Kim E.J., Lee D., Moon S.H., Lee C.H., Kim S.J., Lee J.H., Kim, J.O., Song M., Das B., Clemens J.D., Pape J. W., Nair G.B., Kim D.W. Molecular insights into the evolutionary pathway of Vibrio choleraeO1 atypical El Tor variants. Plos pathogens. 2014, 0(9):e1004384. PMID:25233006
  • Ghosh T.S., Sen Gupta S., Bhattacharya T., Yadav D., Barik A., Chowdhury A., Das B., Mande S.S., Nair G.B. Gut Microbiomes of Indian children of varying nutritional status. PLoS One.2014,9:e95547 PMID:24763225

2013

  • Das B.,Martinez E., Midonet C., and Barre F.X. Integrative mobile elements exploiting Xer recombination. Trends Microbiol. 2014, 21:23-30. PMID:23127381.

2012

  • Pal R.R., Bag S., Dasgupta S., Das B., Bhadra R.K. Functional characterization of the stringent response regulatory gene dksA of Vibrio cholerae and its role in modulation of virulence phenotypes. J. Bacteriol. 2012, 194(20):5638-48.PMID:22904284

2011

  • Das B.,Bischerour J., Barre F.X. VGJFintegration and excision mechanisms contribute to the genetic diversity of Vibrio cholerae epidemic strains. Proc.Natl. Acad. Sci, USA. 2011, 108:2516-2521. PMID:21262799
  • Das B., Bischerour J., Barre F.X. ­Molecular mechanism of acquisition of the cholera toxin genes. Indian J. Med. Res. 2011, 133:195-200.PMID:21415494.
  • Pal R.R., Das B., Dasgupta S., Bhadra R.K. Genetic components of stringent response in Vibrio cholerae. Indian J. Med. Res. 2011,133:212-217. PMID:21415497.
  • Majumdar T., Das B., Bhadra R.K., Dam B., Mazumder S. Complete nucleotide sequence of a quinolone resistance gene (qnrS2) carrying plasmid of Aeromonas hydrophila isolated from fish. Plasmid. 2011, 66:79-84. PMID:21664930.

2010

  • Das B.*, Bischerour J*., Val M.E., Barre F.X. Molecular keys of the tropism of integration of the Cholera Toxin phage. Proc.Natl. Acad. Sci, USA 2010, 107:4377-4382. (Commentary in PNAS). 
  • Halder K., Das B., Nair G. B., Bhadra R.K. Molecular evidences favouring stepwise evolution of Mozambique Vibrio cholerae O1 El Tor hybrid strain. Microbiology(SGM) 2010, 156:99 107. 

2009

  • Das B.,Pal R.R., Bag S., Bhadra R.K. Stringent response in Vibrio cholerae: genetic analysis of spoT gene function and identification of a novel (p)ppGpp synthetase gene. Mol. Microbiol. 2009. 72:380-398. PMID:19298370.

2008

  • Das B.,Bhadra R.K. Molecular characterization of Vibrio cholerae ΔrelA ΔspoT double mutants. Arch. Micrbiol. 2008,189:227-238. PMID:17968531.
  • Shah S., Das B., Bhadra R.K. Functional analysis of the essential GTP-binding protein coding gene cgtA of Vibrio cholerae. J. Bacteriol. 2008, 190:4764-4771. PMID:18456812.
  • Bhowmick R., Ghosal A., Das B., Koley H., Saha D. R., Ganguly S., Nandy R.K., Bhadra R.K., Chatterjee N.S. Intestinal adherence of Vibrio cholerae involves a coordinated interaction between colonization factor GbpA and mucin. Infect. Immun. 2008, 76:4968-4977.PMID:18765724.

2007

  • Das B.,Halder K., Pal P., Bhadra R.K. Small chromosomal integration site of classical CTX prophage in Mozambique Vibrio cholerae O1 biotype El Tor strain. Arch.Micrbiol. 2007, 188:677-683. PMID:17618421.

2006

  • Maiti D., Das B.,Saha A., Nandy R.K., Nair G.B., Bhadra R.K. Genetic organization of the pre-CTX and CTX prophages in the genome of an environmental Vibrio cholerae non-O1, non-O139 strain. Microbiology (SGM) 2006, 152:3633-3641.PMID:17159216.

 

 

Book/Book Chapter

  • eBook (2017): Filamentous Bacteriophage in Bio/Nano/Technology, Bacterial Pathogenesis and Ecology. Edited by: Jasna Rakonjac, Bhabatosh Das, Ratmir Derda. Publisher: Frontiers Media SA. ISBN: 9782889450954
  • Dasgupta S., Das B., Basu P., Bhadra R.K. (2016) Molecular basis of stringent response in Vibrio cholerae (Edited by Frans J. de Bruijn. John Wiley & Sons, Inc, USA). P. 507-516 Aug 2016
  • Nair G.B., Sen Gupta S., Das B.(2014). The “Uncultured” but significant world of the human gut microbiome: New metagenomic insights. In G. P. Talwar and L.M. Srivastava (Eds.), Textbook of Biochemistry and Human Biology (pp. 664-671). Delhi, India: PHI Learning Pvt. Ltd.
  • Das B.and Nair G.B. (2012). The genomics of Cholera. In ‘Genomics Applications for the Developing World’ (Eds. Karen E. Nelson & Barbara jones-Nelson) Springer publication, USA pp. 21-38, DOI: 10.1007/978-1-4614-2182-5_3.
  • Bhadra R.K., Shah S., Das B.(2010). Small Molecule Signaling Systems in Vibrio cholerae.  In ‘Epidemiological and Molecular Aspects on Cholera’ (Eds. Ramamurthy T. & Bhattacharya S. K.). Humana Press, USA, pp. 185-201.
  • Bhadra R.K. and Das B.(2008). Coordinated regulation of gene expression in Vibrio cholerae. In ‘Vibrio cholerae: Genomics and Molecular Biology’ (Eds. Faruque, S.M. & Nair, G.B). Caister Academic Press, UK, pp. 153-167.

 

  • Young Investigator Award (1st prize) from Yakult India Microbiota and Probiotic Science Foundation, 2016
  • CNRS Post Doctoral Research Fellowship, Govt. of France
  • CSIR Research Fellowship, Govt. of India
  • ICMR Research Fellowship, Govt. of India
  • IIT Fellowship, IIT-Kharagpur, Govt. of India

Editorial Board Member

  • Frontiers in Microbiology

            (Nature publishing group)

  • Microbial Ecology

           (Springer)