People : Scientific : Faculty & Scientists

DR. AMIT AWASTHI, Ph. D. 

Associate Professor
+91-129-2876482  (+91-129-2876482)
aawasthi [at] thsti [dot] res [dot] in
Instructor (Junior Faculty)Harvard Medical School, MA, USA
Postdoctoral Training from Brigham and Womens Hospital and Harvard Medical School, Boston, MA, USA
Ph.D in Immunology from National Center for Cell Science, Pune
M.Sc. in Biotechnology from Jiwaji University Gwalior

Present Research Interest

  • Present research interest lies in the area of understanding the interplay between effector and regulatory T cells in IBD and gut infection.
  • Understanding T cell differentiation pathways in immunity, autoimmunity and cancer immunity.
  • Understanding the metabolic regulation of T cells during their differentiaon.
  • Identify and understand the role of check point inhibitors in cancer immunity.
  • Understanding transcriptional regualtions of Th9 and Th17 cells. 

 

 

  1. Awasthi A, Mathur R, Khan A, Joshi BN, Jain N, Sawant S, Boppana R, Mitra D, Saha B. CD40 signaling is impaired in L. major-infected macrophages and is rescued by a p38MAPK activator establishing a host-protective memory T cell response. Journal of Experimental Medicine 2003; 197: 1037-43.
  2. Mathur RK, Awasthi A, Wadhone P, Ramanamurthy B, Saha B. Reciprocal CD40 signals through p38MAPK and ERK-1/2 induce counteracting immune responses. Nature Medicine 2004; 10: 540-4.
  3. Bodas M, Jain N, Awasthi A, Martin S, Penke Loka RK, Dandekar D, Mitra, Saha B. Inhibition of IL-2 induced IL-10 production as principle of phase-specific immunotherapy. Journal of Immunology 2006; 177: 4636-43.
  4. Korn T, Reddy J, Gao W, Bettelli E, Awasthi A, Petersen TR, Backstrom BT, Sobel RA, Wucherpfenning KW, Strom TB, Oukka M, Kuchroo VK. Myelin-specific regulatory T cells accumulate in the central nervous system, but fail to suppress pathogenic effector T cells at the peak of autoimmune inflammation. Nature Medicine 2007; 13: 423-31.
  5. Korn T, Bettelli E, Gao W, Awasthi A, Jäger A, Strom TB, Oukka M, Kuchroo VK. IL-21 initiates an alternate pathway to induce proinflammatory Th17 cells. Nature 2007; 448: 484-7. 
  6. Awasthi A, Carrier Y, Peron JP, Bettelli E, Kamanaka M, Flavell RA, Kuchroo VK, Oukka M,  Weiner HL. A dominant function for interleukin 27 in generating interleukin 10–producing anti-inflammatory T cells. Nature Immunology 2007; 12:1380-9. 
  7. Awasthi A*,Dardalhon V*, Kwon H, Galileos G, Gao W, Strom TB, Oukka M, Kuchroo VK. IL-4 inhibits TGF-b-induced-Foxp3+T cells and generates a Foxp3- IL-10/IL-9 T cell population. Nature Immunology 2008; 9:1347-55 [*Equal first authorship].
  8. Korn T, Mitsdoerffer M, Croxford AL, Awasthi A, Dardalhon VA, Galileos G, Vollmar P, Stritesky GL, Kaplan MH, Waisman A, Kuchroo VK, Oukka M. IL-6 controls Th17 immunity in vivo by inhibiting the conversion of conventional T cells into Foxp3+ T-regs. Proceeding of National Academy of Science USA 2008; 105:18460-5.
  9. Awasthi A, Riol-Blanco L, Jäger A, Korn T, Pot C, Galileos G, Bettelli E, Kuchroo VK, Oukka M. Cutting Edge: IL-23 receptor reporter mice reveal distinct populations of IL-17 producing cells. Cutting Edge: Journal of Immunology 2009; 182: 5904-08.
  10. Elyaman W, Bradshaw EM, Uyttenhove C, Dardalhon V, Awasthi A, Imitola J, Bettelli E, Oukka M, van Snick J, Renauld JC, Kuchroo VK, Khoury SJ. Reciprocal role of IL-9 in regulation of immunity: Enhancement of T-regs functions and Th17 differentiation. Proceeding of National Academy of Science USA 2009; 106:12885-90.
  11. Pot C, Jin H, Awasthi A, Liu SM, Lai CY, Madan R, Sharpe AH, Karp CL, Miaw SC, Ho IC, Kuchroo VK. Cutting Edge: IL-27 Induces the Transcription Factor c-Maf, Cytokine IL-21, and the Costimulatory Receptor ICOS That Coordinately Act Together to Promote Differentiation of IL-10-Producing Tr1 Cells. Cutting Edge: Journal of Immunology 2009; 183: 797-801.
  12. Awasthi A, Kuchroo VK. IL-17A directly inhibits TH1 cells and thereby regulates development of intestinal inflammation. Nature Immunology 2009; 10:568-70.
  13. Awasthi A, Kuchroo VK. Yin and Yang of Follicular T cell Development. Science 2009; 325:953-5.
  14. Riol-Blanco L,Lazarevic V, Awasthi A, Mitsdoerffer M, Wilson BS, Waisman A, Kuchroo VK, Glimcher LH& Oukka M. IL-23 receptor regulates unconventional IL-17-producing T cells that control early stages of infection. Journal of Immunology 2010; 184:1710-20.
  15. Putheti P, Awasthi A, Gao W & Strom TB. IL-4 and TGF-binduce human memory T cells to become IL-9+CD4+ T cells. PlosOne 2010; 5:e8706.
  16. Awasthi A, Kuchroo VK. Value added, Neural progenitor cells suppress inflammation and autoimmunity. Immunity 2011; 35:156-7.
  17. Lee Y*,Awasthi A*, Yosef N, Quintana F, Xiao S, Kunder S, Regev A, Sobel R, Kuchroo VK. Induction and molecular signature of pathogenic TH17 cells.Nature Immunology 2012; 13:991-9[Shared first and Co-senior author].
  18. Yosef N, Shalek AK, Gaublomme J, Jin H, Lee Y, AwasthiA, Wu C, KarwaczK, Park H, Kuchroo VK, Regev A. Reconstruction of the dynamic regulatory network that controls Th17 cell differentiation by systematic perturbation in primary cells.Nature 2013, 25;496:461-8.
  19. Tyagi RK, Garg NK, Jadon R, Sahu T, Karare OP, Awasthi A, Marepally SK, Elastic liposome mediated transdermal immunization enhanced the immunogenicity of P. falciparum surface antigen, MSP-1. Vaccine 2015, 33: 4630-8
  20. Tyagi R, Garg N, Dalai S, Awasthi A. Transdermal immunization of P. falciparum surface antigen (MSP-119) via elastic liposomes confers robust immunogenicity. Human Vaccine & Therapeutics 2016 Jan 25:0.
  21. Rampal R, Awasthi A, Ahuja V. Retinoic acid primed human dendritic cells promote gut-tropism, inhibit Th9 cells and induce Th1/Th17 cell differentiation. J Leukoc Biol 2016; jlb.3VMA1015-476R
  22. Chihara N, Madi A, Karwacz K, Awasthi A, Kuchroo VK. Differentiation and characterization of Tr1 cells. Current Protocols in Immunol 2016; 113:3.27.1-3.27.10
  23. Khalili H, Malik S, Ananthakrishnan A, Garber J, Higuchi L, Joshi A, Peloquin J, Richter J, Stewart K, Curhan G, Awasthi A*, Yajnik V, Chan A. Dietary Sodium and Potassium Intake, Immune Tolerance and Risk of Crohn's Disease and Ulcerative Colitis. Frontiers in Immunology 2016;7:554. doi: 10.3389/fimmu.2016.00554(*Shared Co-senior author)
  24. Gu G, HuangY, WuC, GuoZ, Awasthi A*,He X, Differential expression of long noncoding RNAs during cardiac allograft rejection. Transplantation 2017,101:83-91.doi: 10.1097/TP.0000000000001463(*SharedCo-senior author)
  25. Malik S, Dardhalon V, Awasthi A. Role of Th9 cells in EAE and IBD: Lessons learnt from adoptive transfer experiments. Methods in Mol Biol 2017, pp.201-216, DOI: 10.1007/978-1-4939-6877-0_16·
  26. Karwacz K, Miraldi ER, Pokrovskii M, Madi A, Yosef N, Wortman I, Chen X, Watters A, Carriero N, Awashti A, Regev A, Bonneau R, Littman D, Kuchroo VK. Critical role of IRF1 and BATF in preparing the chromatin landscape during Tr1 differentiation. Nature Immunology 2017, 18(4):412-421. doi: 10.1038/ni.3683.
  27. Malik S, Sadhu S, Elesela S, Pandey RP, Chawla AS, Sharma D, Panda L, Rathore D, Ghosh B, Ahuja V, Awasthi ATranscription factor Foxo1 is essential for IL-9 induction in T helper cells. Nature Communications, 2017 Oct 9;8(1):815. doi: 10.1038/s41467-017-00674-6

Reviews: 

  1. Awasthi A, Mathur RK, Saha B. Immune response to Leishmania infection. Indian Journal of Medical Research 2004; 119, 238-58
  2. Mathur RK, Awasthi A, Saha B. The conundrum of CD40 function: Host-protection and disease-promotion? Trends in Parasitology 2006; 22: 117-22.
  3. Awasthi A, Murugaiyan G, Kuchroo VK. Interplay between effector Th17 and regulatory T cells.  Journal of Clinical Immunology 2008; 28:660-70.
  4. Awasthi A, Kuchroo VK. Th17 cells: from precursors to players in inflammation and infection. International Immunology 2009; 21:489-98.
  5. Pot C, Apetoh L, Awasthi A, Kuchroo VK. Interleukin-27 as a novel cytokine to promote interleukin-10-producing regulatory type 1 (Tr1) cells development: molecular mechanisms and clinical implications. Journal of Interferon Cytokine Research 2010; 30: 381-8.
  6. Awasthi A, Kuchroo VK. IL-17 and Th17 cells in immunity and autoimmunity. Epublication Focis 2011; May issue.
  7. Pot C, Apetoh L, Awasthi A, Kuchroo VK. Induction of regulatory Tr1 cells and inhibition of TH17 cells by IL-27. Seminar In Immunology 2011; 23:438-45.
  8. Kuchroo VK,Awasthi A. Emerging new roles of Th17 cells.European Journal of Immunology 2012; 42(9):2211-4.
  9. Malik S, Muzamil Want, Awasthi A, Emerging role of gamma-delta T cells in tissue inflammation in EAE. Frontiers of Immunology 2016; 7:14.
Reviews
  1. Awasthi AMathur RK, Saha B. Immune response to Leishmania infection. Indian Journal of Medical Research 2004; 119, 238-58.
  2. Mathur RK, Awasthi A, Saha B. The conundrum of CD40 function: Host-protection and disease-promotion? Trends in Parasitology 2006; 22: 117-22.
  3. Awasthi A, Murugaiyan G, Kuchroo VK. Interplay between effector Th17 and regulatory T cells.  Journal of Clinical Immunology 2008; 28:660-70.
  4. Awasthi A, Kuchroo VK. Th17 cells: from precursors to players in inflammation and infection. International Immunology 2009; 21:489-98.
  5. Pot C, Apetoh L, Awasthi A, Kuchroo VK. Interleukin-27 as a novel cytokine to promote interleukin-10-producing regulatory type 1 (Tr1) cells development: molecular mechanisms and clinical implications. Journal of Interferon Cytokine Research 2010; 30: 381-8.
  6. Awasthi A, Kuchroo VK. IL-17 and Th17 cells in immunity and autoimmunity. Epublication Focis 2011; May issue.
  7. Pot C, Apetoh L, Awasthi A, Kuchroo VK. Induction of regulatory Tr1 cells and inhibition of TH17 cells by IL-27. Seminar In Immunology 2011; 23:438-45.
  8. Kuchroo VK,Awasthi A. Emerging new roles of Th17 cells.European Journal of Immunology 2012; 42(9):2211-4.
  9. Malik S, Muzamil Want, Awasthi A, Emerging role of gamma-delta T cells in tissue inflammation in EAE. Frontiers of Immunology 2016; 7:14.
Book Chapters
  1. Saha B, Awasthi A, Mathur R.  Reciprocal CD40 signaling through p38MAP kinase and ERK1/2 in Leishmania infection. Deep Roots Open Skies: New Biology in India 2004. p. 167-72.
  2. Awasthi A, Leu SM, Kuchroo VK. Cytokines and immune system. HLA system in Medicine and Biology 2010 Jaypee Brothers Medical Publishers Ltd.; 1st ed. (Book Chapter) p. 19-41.
  3. Awasthi A and Kuchroo VK. From TH1/TH2 paradigm to TH17 cells: Le roi est mort vive le roi,TH17 Cells in Health and Disease 2010. Springer (Book Chapter) p. 3-25. 
  1. 2001                      Junior Research Fellowship, Department of Biotechnology, Government of India.
  2. 2003                     Senior Research Fellowship, Council of Scientific & Industrial Research, Government of India.
  3. 2004                     G.P. Talvar Young Scientist Award of Indian Immunology Society, India
  4. 2007                     Second place (Trophy and cash prize) at 2nd Abstract Competition at MedImmune, Inc. Gaithersburg, MD, USA.
  5. 2008                     Tykeson Fellows Conference of Multiple Sclerosis Society, Nov 4-7, 2008 Chicago, USA.
  6. 2010                     Tykeson Fellows Conference of Multiple Sclerosis Society, June 4-7, 2010 San Antonio, USA.
  7. 2007–2010          National Multiple Sclerosis Society Postdoctoral Fellowship, National Multiple Sclerosis Society, New York, USA
  8. 2009                      Travel Award, Federation of Clinical Immunology Society (Focis)
  9. 2011                     Research pilot grant from Crohn’s and Colitis Foundation of America, New York, USA
  10. 2011                     Ramalingaswami Fellowship from Department of Biotechnology, India
  11. 2011                     Innovative Young Biotechnologist Award from Department of Biotechnology, Govt. of India.
  12. 2012                     DBT-Wellcome India Alliance Intermediate Fellowship.
  13. 2012                     Young scientist platinum jubilee award in biological sciences, National Academy of Sciences of India.
  14. 2016                     AAI Early Career Faculty Travel Grantfor attending AAI annual meeting at Seattle, Washington  
  15. 2016                     AAI Travel Grant for the International Congress of Immunology 2016 (ICI 2016), Melbourne Australia
  16. 2017                     G.P. Talwar Mid Career Scientist Award of Indian Immunology Society India
  17. 2017                     AAI Early Career Faculty Travel Grantfor attending AAI annual meeting at Washington D.C. 

Professional Societies:

       2004–Present     Indian Immunology Society Member (IIS)

       2015-Present       American Association of Immunologist (AAI)

       2017-present        Elected executive council member of Indian Immunology Society Member (IIS)   

       2017-present        Nominated member of Molecular Immunology Forum (MIF)

 

Invited Presentations:

1. 2005            Oral presentation at Department of Pathology, University of Chicago (May 23, 2005) on “Biology and cell signaling in parasitic infection”.

2. 2008            Invited speaker at Indian Immunology Society, Bhuvaneshwar, India.

3. 2009            Young Researchers Travel AwardFederation of Clinical Immunology Societies.Focis, San Francisco, USA.

4. 2012             Invited speaker and panelist, Young Investigator Meeting, Boston, USA

5. 2012             Invited talk at Children Hospital and Harvard Medical School, Boston, MA, USA

6. 2012             Special lecture at 4th Women Scientist Program at C. R. Rao AIMCIS, Hyderabad

7. 2013            Oral presentation at Harvard-AIIMS GI Immunology Conference, New Delhi

8. 2013             Invited talk at IBD conference at G.B. Pant Hospital, New Delhi

9. 2013             Invited presentation at Indian Institute of Science, Education and Research (IISER), Bhopal.

10. 2014          Invited as Young Investigator at 6th Young Investigator Meeting at Hyderabad.

11. May 13, 2014 Invited speaker at Massachusetts General Hospital, Harvard Medical School “ Induction and development of pathogenic Th17 cells and their regulation”

12. May 15, 2014 Invited speaker at Oakland University, Michigan “ Induction and development of pathogenic Th17 cells and their regulation”

13. March 27, 2015 Invited speaker at Biosparks, School of Life Sciences, Jawaharlal Nehru University (JNU). New Delhi

14. Oct. 9-11, 2015, Invited lead talk at annual meeting of Indian Immunology Society, Patna, India

15. Oct 14, 2015, Invited as an International speaker at Academy of Immunology and Microbiology (AIM), IBS, South Korea

16. 13-17 May, 2016Poster presentation atannual meeting of American Association of Immunologists at Seattle, Washington, USA

17. 01 Aug, 2016. Invited speaker at Emory Vaccine Center, Atlanta USA“Understanding transcriptional regulation of IL-9 in Th9 and Th17 cells in health and diseases”

18. 02 Aug, 2016. Invited speaker at Nebraska Medical Center, Omaha, USA. “Understanding transcriptional regulation of IL-9 in Th9 and Th17 cells in health and diseases.”

19. 21-26 Aug, 2016 Oral Presentation at International Congress of Immunology, Melbourne, Australia.

20. 12-16 May, 2017 Poster presentation at annual meeting of American Association of Immunologists atWashington D.C., USA


MR. SHARANABASAVA
ASSISTANT VACCINE TECHNOLOGIST

MR. MANAS RANJAN TRIPATHY
LAB TECHNICIAN

MS. SUYASHA ROY
PH.D STUDENT

MR. RAJ KUMAR
LAB TECHNICIAN

DR. ZAIGHAM ABBAS RIZVI
RESEARCH ASSOCIATE

MS. SAKSHI MALIK
PH.D STUDENT

MR. RAJDEEP DALAL
PH.D STUDENT

MR. SRIKANT SADHU
PH.D STUDENT