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DR. AJAY KUMAR, Ph.D. Life Sciences, Guru Nanak Dev University, Amritsar  

Scientist D
+91-129-2876488 
a.kumar [at] thsti [dot] res [dot] in
M.Sc. Molecular Biology and Biochemistry, Guru Nanak Dev University, Amritsar
B.Sc. Biology, Guru Nanak Dev University, Amritsar


Broad interest of my research includes identifying pathways or proteins involved in early stages of progression of metabolic diseases including Type 2 diabetes, obesity, etc. I and my team members are working closely with the mathematics team at DDRC to identify drug targets from high throughput proteomic and metabolomic datasets. Our focus is to study context and time specific changes in the proteome and metabolome of different tissue types from diseased animal models compared to their normal counterparts. The putative drug targets are validated by employing different molecular approaches including CRISPR-Cas9 based genome editing, siRNA known-downs, protein over-expression employing lentivirus based or gateway cloning strategies, etc. We are specifically focused on developing in-house expertise on designing CRISPR-Cas9 based genome editing tools and exploring their diverse applications in drug target discovery. 

 

Previous Jobs/Trainings

  • Scientist B at Immunology group, ICGEB, New Delhi (2011-2016)
  • Research Scientist at Immunology Group, ICGEB, New Delhi (2010-2011)

 

  • Defining the Akt1 interactome and its role in regulating the cell cycle. Scientific Reports 2018 8: 1303
  • Rb interactome data and its modulations during cell cycle progression in HEK 293 cells. Data in Brief 2018 17:604-609
  • Restoring calcium homeostasis in diabetic cardiomyocytes: An investigation through mathematical modelling. Molecular BioSystems  2017 DOI:10.1039/C7MB00264E.
  • Dataset to delineate changes in association between Akt1 and its interacting partners as a function of active state of Akt1 protein. Data in Brief 2017 13:187-191.
  • Defining the Akt1 Interactome Data and Delineating Alterations in its Composition as a function of Cell Cycle Progression. Data in Brief 2017 11: 252-257
  • Dataset generated using hyperplexing and click chemistry to monitor temporal dynamics of newly synthesized macrophage secretome post infection by mycobacterial strains. Data in Brief 2016 9: 349–354.
  • M235T polymorphism in the AGT gene and A/GI8-83 substitution in the REN gene correlate with end stage renal disease. Nephron Clinical Practice 2015 DOI: 10.1159/000370074.
  • Depleted TGF-β₁levels in end stage renal disease patients from North India. Gene 2014 534: 440-443.
  • Genomic Diversity and Affinities in population groups of North West India: An analysis of Alu insertion and a single nucleotide polymorphism. Gene 2012 511: 293–299.
  • Tumor Necrosis Factor-α and Transforming growth factor-β1 polymorphisms in Bronchial Asthma. Indian Journal of Medical Sciences 2008 62: 323-330.
  • Transforming Growth Factor-beta1 gene polymorphism in tuberculosis patients. International Journal of Human Genetics 2007 7: 249-252.
  • Association study of Angiotensin-Converting Enzyme Gene Ins/Del polymorphism with hypertension in Punjabi population. International Journal of Human Genetics 2006 6: 317-321.
  • Angiotensin-I converting enzyme I/D polymorphism and Diabetic nephropathy in North India. International Journal of Human Genetics 2005 5: 279-283.
  • Ajay Kumar, Shilpa Jamwal, Suruchi Aggarwal, Kanury VS Rao, Amit Kumar Yadav. Method of hyperplexing in mass spectrometry to elucidate temporal dynamics of proteome. (Ref No. 201611029904)
  • Travel grant awarded by Translational Health Science and Technology Institute (THSTI), Faridabad, India to attend EMBL course on “Quantitative Proteomics: Strategies and Tools to Probe Biology” organized by European Molecular Biology Laboratory (EMBL) at EMBL headquarters, Heidelberg, Germany from 19th – 23rd June, 2017. 

  • Travel grant awarded by Council for Scientific and Industrial Research (CSIR), New Delhi, India to attend European Molecular Biology Organization (EMBO) Practical course on “Gene Expression Analysis in Diagnostic Medicine” held at St. Luke’s Medical Centre, Quezon City, Philippines from 17th-26th September, 2006.

  • Travel Grant awarded by National Centre for Biological Sciences (NCBS), Bangalore, India, to attend International Symposium on human origins and genetics entitled “Genes, Evolution and Complex diseases” held at Bangalore, Indiafrom 17th-19th February, 2005.

Past Lab Members

Dr. Alka Kaushal (Research Associate I)

Ms. Pooja Chaudhary (Junior Research Fellow)

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